In Vivo Calcium Imaging During Axon Degeneration in Zebrafish

In vivo calcium imaging in zebrafish provides the ability to investigate calcium dynamics within neurons. Utilizing genetically encoded calcium sensors it is possible to monitor calcium signals within a single axon during axon injury and degeneration with high temporal and spatial resolution. Here w...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2143; p. 263
Main Author Vargas, Mauricio E
Format Journal Article
LanguageEnglish
Published United States 2020
Subjects
Animals
Animals, Genetically Modified
Axons - chemistry
Axons - physiology
Axons - ultrastructure
Calcium - analysis
Calcium Signaling
Calcium-Binding Proteins - analysis
Calcium-Binding Proteins - genetics
Cytoplasm - chemistry
Female
Genes, Reporter
Green Fluorescent Proteins - analysis
Green Fluorescent Proteins - genetics
Intracellular Calcium-Sensing Proteins - analysis
Intravital Microscopy - instrumentation
Intravital Microscopy - methods
Male
Mitochondria - chemistry
Time-Lapse Imaging - instrumentation
Time-Lapse Imaging - methods
Wallerian Degeneration - metabolism
Wallerian Degeneration - pathology
Zebrafish - embryology
GCaMP
Axon
Confocal microscopy
Zebrafish
Calcium
Online AccessGet more information

Cover

More Information
Summary:In vivo calcium imaging in zebrafish provides the ability to investigate calcium dynamics within neurons. Utilizing genetically encoded calcium sensors it is possible to monitor calcium signals within a single axon during axon injury and degeneration with high temporal and spatial resolution. Here we will describe in vivo, time-lapse confocal imaging methods of calcium imaging. Imaging of calcium dynamics with genetically encoded calcium sensors (GECS) within living axons can serve as a method to assess axonal physiology and effects of pharmacologic and genetic manipulation, as well as characterize responses to different injury models.
ISSN:1940-6029
DOI:10.1007/978-1-0716-0585-1_19