Immunity-Associated Programmed Cell Death as a Tool for the Identification of Genes Essential for Plant Innate Immunity

Plants have evolved a sophisticated innate immune system to contend with potential infection by various pathogens. Understanding and manipulation of key molecular mechanisms that plants use to defend against various pathogens are critical for developing novel strategies in plant disease control. In...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1743; p. 51
Main Authors Zhou, Bangjun, Zeng, Lirong
Format Journal Article
LanguageEnglish
Published United States 2018
Subjects
Apoptosis - genetics
Apoptosis - immunology
Gene Expression Regulation, Plant
Gene Knockout Techniques
Gene Silencing
Genes, Essential
Host-Pathogen Interactions - genetics
Host-Pathogen Interactions - immunology
Phenotype
Plant Diseases - genetics
Plant Diseases - immunology
Plant Diseases - microbiology
Plant Diseases - virology
Plant Immunity - genetics
Plants - genetics
Plants - immunology
Seedlings - genetics
Seedlings - immunology
Transformation, Genetic
Agro-mediated transient expression
Nicotiana benthamiana
Ubiquitination
Programmed cell death
Plant innate immunity
Virus-induced gene silencing
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Summary:Plants have evolved a sophisticated innate immune system to contend with potential infection by various pathogens. Understanding and manipulation of key molecular mechanisms that plants use to defend against various pathogens are critical for developing novel strategies in plant disease control. In plants, resistance to attempted pathogen infection is often associated with hypersensitive response (HR), a form of rapid programmed cell death (PCD) at the site of attempted pathogen invasion. In this chapter, we describe a method for rapid identification of genes that are essential for plant innate immunity. It combines virus-induced gene silencing (VIGS), a tool that is suitable for studying gene function in high-throughput, with the utilization of immunity-associated PCD, particularly HR-linked PCD as the readout of changes in plant innate immunity. The chapter covers from the design of gene fragment for VIGS, the agroinfiltration of the Nicotiana benthamian plants, to the use of immunity-associated PCD induced by twelve elicitors as the indicator of activation of plant immunity.
ISSN:1940-6029
DOI:10.1007/978-1-4939-7668-3_5