Singlet Oxygen and Protochlorophyllide Detection in Arabidopsis thaliana

Since the recognition of the reactive oxygen species singlet oxygen ( O ) as a versatile signal that induces various stress responses, the mechanisms underlying O -induced signaling transduction pathways have become the subject of much current research. This in turn highlights the need for reliable...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2202; p. 63
Main Authors Wang, Liangsheng, Kleine, Tatjana
Format Journal Article
LanguageEnglish
Published United States 2021
Subjects
Arabidopsis - metabolism
Fluorescent Dyes - metabolism
Light
Oxygen - analysis
Oxygen - metabolism
Photosensitizing Agents - metabolism
Protochlorophyllide - metabolism
Reactive Oxygen Species - metabolism
Signal Transduction
Single-Cell Analysis - methods
Singlet Oxygen - analysis
Singlet Oxygen - metabolism
Protochlorophyllide
Singlet oxygen
Arabidopsis
Fluorescent mutant
Chloroplast
Singlet Oxygen Sensor Green (SOSG)
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Summary:Since the recognition of the reactive oxygen species singlet oxygen ( O ) as a versatile signal that induces various stress responses, the mechanisms underlying O -induced signaling transduction pathways have become the subject of much current research. This in turn highlights the need for reliable detection methods for O . Here we describe a protocol for the detection of O using a commercially available fluorescent probe (Singlet Oxygen Sensor Green) and provide a simple method for direct visualization and quantification of the O -evolving photosensitizer protochlorophyllide in the Arabidopsis fluorescent mutant.
ISSN:1940-6029
DOI:10.1007/978-1-0716-0896-8_5