Quantitative Phosphoproteomic Using Titanium Dioxide Micro-Columns and Label-Free Quantitation

Phosphorylation events are important during cellular function. Analysis of phosphorylation in complex samples has been extensively studied using large-scale phosphopeptide enrichment methods. Quantitative analysis of the enriched phosphopeptides is subsequently performed using label-based methodolog...

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Published inMethods in molecular biology (Clifton, N.J.) Vol. 1977; p. 35
Main Authors Barrios-Llerena, Martin E, Le Bihan, Thierry
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
Chromatography, Affinity - methods
Data Analysis
Phosphopeptides - chemistry
Phosphopeptides - metabolism
Phosphoproteins - chemistry
Phosphoproteins - metabolism
Phosphorylation
Proteomics - methods
Titanium - chemistry
Label-free quantitation
Titanium dioxide chromatography (TiO2)
Mass spectrometry
Phosphopeptide enrichment
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Abstract Phosphorylation events are important during cellular function. Analysis of phosphorylation in complex samples has been extensively studied using large-scale phosphopeptide enrichment methods. Quantitative analysis of the enriched phosphopeptides is subsequently performed using label-based methodologies (e.g., SILAC, iTRAQ, and others). Here we describe the protocol for the quantitative analysis of phosphopeptides, enriched with titanium dioxide micro-column, using an intensity-based label-free quantitation.
AbstractList Phosphorylation events are important during cellular function. Analysis of phosphorylation in complex samples has been extensively studied using large-scale phosphopeptide enrichment methods. Quantitative analysis of the enriched phosphopeptides is subsequently performed using label-based methodologies (e.g., SILAC, iTRAQ, and others). Here we describe the protocol for the quantitative analysis of phosphopeptides, enriched with titanium dioxide micro-column, using an intensity-based label-free quantitation.
Author Barrios-Llerena, Martin E
Le Bihan, Thierry
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  fullname: Barrios-Llerena, Martin E
  email: martin.barriosllerena@kaust.edu.sa
  organization: Proteomics and Mass Spectrometry, Bioscience Core Labs, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia. martin.barriosllerena@kaust.edu.sa
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  givenname: Thierry
  surname: Le Bihan
  fullname: Le Bihan, Thierry
  organization: Synthetic and Systems Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK
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Keywords Label-free quantitation
Titanium dioxide chromatography (TiO2)
Mass spectrometry
Phosphopeptide enrichment
Language English
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PublicationTitle Methods in molecular biology (Clifton, N.J.)
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Snippet Phosphorylation events are important during cellular function. Analysis of phosphorylation in complex samples has been extensively studied using large-scale...
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StartPage 35
SubjectTerms Chromatography, Affinity - methods
Data Analysis
Phosphopeptides - chemistry
Phosphopeptides - metabolism
Phosphoproteins - chemistry
Phosphoproteins - metabolism
Phosphorylation
Proteomics - methods
Titanium - chemistry
Title Quantitative Phosphoproteomic Using Titanium Dioxide Micro-Columns and Label-Free Quantitation
URI https://www.ncbi.nlm.nih.gov/pubmed/30980321
Volume 1977
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