Quantitative Phosphoproteomic Using Titanium Dioxide Micro-Columns and Label-Free Quantitation

Phosphorylation events are important during cellular function. Analysis of phosphorylation in complex samples has been extensively studied using large-scale phosphopeptide enrichment methods. Quantitative analysis of the enriched phosphopeptides is subsequently performed using label-based methodolog...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1977; p. 35
Main Authors Barrios-Llerena, Martin E, Le Bihan, Thierry
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
Chromatography, Affinity - methods
Data Analysis
Phosphopeptides - chemistry
Phosphopeptides - metabolism
Phosphoproteins - chemistry
Phosphoproteins - metabolism
Phosphorylation
Proteomics - methods
Titanium - chemistry
Label-free quantitation
Titanium dioxide chromatography (TiO2)
Mass spectrometry
Phosphopeptide enrichment
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Summary:Phosphorylation events are important during cellular function. Analysis of phosphorylation in complex samples has been extensively studied using large-scale phosphopeptide enrichment methods. Quantitative analysis of the enriched phosphopeptides is subsequently performed using label-based methodologies (e.g., SILAC, iTRAQ, and others). Here we describe the protocol for the quantitative analysis of phosphopeptides, enriched with titanium dioxide micro-column, using an intensity-based label-free quantitation.
ISSN:1940-6029
DOI:10.1007/978-1-4939-9232-4_4