Measuring Circadian Rhythms in Human Cells

Human cells, especially primary fibroblasts from skin punch biopsy, have emerged over the last decade as powerful, unlimited, and easily accessible resources that bridge the gap between animal models and human subjects in basic as well as clinical research. The cells also retain molecular circadian...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2130; p. 53
Main Authors Du, Ngoc-Hien, Brown, Steven A
Format Journal Article
LanguageEnglish
Published United States 2021
Subjects
Cell Line
Cells, Cultured
Circadian Clocks
Fibroblasts - metabolism
Humans
Primary Cell Culture - methods
Skin - cytology
Circadian rhythm measurement
Human primary skin fibroblasts
Human skin punch biopsy
Stable cell line generation
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Summary:Human cells, especially primary fibroblasts from skin punch biopsy, have emerged over the last decade as powerful, unlimited, and easily accessible resources that bridge the gap between animal models and human subjects in basic as well as clinical research. The cells also retain molecular circadian clocks that reflect subject-specific differences in circadian physiology, and the cellular rhythms can be measured easily in large scale. This is a series of protocols that describes the procedure to measure circadian rhythms in these cells, starting from deriving fibroblasts from skin punch biopsy, to generation of stable cells expressing a circadian reporter, and finally measurement of cellular rhythms in large scale.
ISSN:1940-6029
DOI:10.1007/978-1-0716-0381-9_4