Assaying D-Body Amino Acids as D-Alanine and Amino Acid Racemase (AARase) Activity Using NADH Oxidoreduction Enzymic System

Fermented foods produced using edible microorganisms have been consumed worldwide since ancient times. Microorganisms produce some healthy functional substances. Recently, many people have been attracted to the notion that D-body amino acids have healthy functional effects. D-body amino acids conver...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1887; p. 23
Main Authors Matsumoto, Natsuki, Kanauchi, Makoto
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
D-Body amino acid
D-Alanine
D-Amino acid oxidase
Oxidoreduction system
Amino acid racemase
Fermented food
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Summary:Fermented foods produced using edible microorganisms have been consumed worldwide since ancient times. Microorganisms produce some healthy functional substances. Recently, many people have been attracted to the notion that D-body amino acids have healthy functional effects. D-body amino acids convert L-body amino acid proteolysis from a substrate such as foods during fermentation. This chapter presents a description of methods used for D-alanine assays in the solution as medium and AARase assays in lactic acid bacteria (LAB) cells for isolation of high AARase-containing strains using D-amino acid oxidase and lactic acid dehydrogenase via a NADH oxidoreduction system.
ISSN:1940-6029
DOI:10.1007/978-1-4939-8907-2_3