Bacterial Group II Introns: Identification and Mobility Assay

Group II introns are large catalytic RNAs and mobile retroelements that encode a reverse transcriptase. Here, we provide methods for their identification in bacterial genomes and further analysis of their splicing and mobility capacities.

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1400; p. 21
Main Authors Toro, Nicolás, Molina-Sánchez, María Dolores, Nisa-Martínez, Rafael, Martínez-Abarca, Francisco, García-Rodríguez, Fernando Manuel
Format Journal Article
LanguageEnglish
Published United States 2016
Subjects
Bacteria - genetics
DNA Transposable Elements
DNA, Bacterial
Genome, Bacterial
Genomics - methods
Introns
Open Reading Frames
RNA Splicing
RNA, Bacterial
Catalytic RNAs
Reverse transcriptase
RNA
Retroelements
Mobile DNA
Group II introns
Maturase
Ribozymes
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Summary:Group II introns are large catalytic RNAs and mobile retroelements that encode a reverse transcriptase. Here, we provide methods for their identification in bacterial genomes and further analysis of their splicing and mobility capacities.
ISSN:1940-6029
DOI:10.1007/978-1-4939-3372-3_2