Using IC-Tagging Methodology for Production and Purification of Epitope-Loaded Protein Microspheres for Vaccination

Particulate material is more efficient in eliciting immune responses. Here we describe the production of microspheres formed by protein muNS-Mi from avian reoviruses, loaded with foreign epitopes by means of IC-Tagging, for their use as vaccines.

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1349; p. 25
Main Authors Barreiro-Piñeiro, Natalia, Menaya-Vargas, Rebeca, Brandariz-Núñez, Alberto, Otero-Romero, Iria, Lostalé-Seijo, Irene, Benavente, Javier, Martínez-Costas, José M
Format Journal Article
LanguageEnglish
Published United States 2016
Subjects
Animals
Birds - immunology
Birds - virology
Epitopes - immunology
Fluorescence
Microspheres
Orthoreovirus, Avian - drug effects
Orthoreovirus, Avian - pathogenicity
Vaccination - methods
Viral Nonstructural Proteins - immunology
Microspheres
Adjuvants
Avian reovirus
Vaccines
IC-Tagging
Particulate material
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Summary:Particulate material is more efficient in eliciting immune responses. Here we describe the production of microspheres formed by protein muNS-Mi from avian reoviruses, loaded with foreign epitopes by means of IC-Tagging, for their use as vaccines.
ISSN:1940-6029
DOI:10.1007/978-1-4939-3008-1_2