Response of creatine kinase (CK) levels in diabetics Wistar rats after acute physical exercise

• Published in: Journal of exercise physiology online Vol. 16; no. 2; pp. 99 - 111
• Main Authors: Costa, Jr., Marcelo, Spagnol, Alexandre Roveratti, Pereira, Aron da Silva, Novais, Iane de Paiva, de Castro, Marcela Rodrigues, Anaruma, Chadi Pellegrini, da Silva, Carmem Peres Valgas, Luciano, Eliete
• Format: Journal Article
• Language: English
• Published: American Society of Exercise Physiologists 01.04.2013
• Subjects: Creatine kinase; Diabetes; Exercise; Physiological aspects; Physiological research
• ISSN: 1097-9751; 1097-9751

This study examined the effects of acute exercise on hyperglycemia and kinetic behavior of creatine kinase (CK) in diabetic rats. Wistar rats were distributed into two groups: Exercise Control (C) and Exercise Diabetic (D). Diabetes mellitus was induced by alloxan monohydrate Sigma® (32 mg x [kg.sup.-1] of body weight). After 7 days, a glucose test was carried out. Animals with values equal to or higher than 200 mg x [dL.sup.-1] were considered diabetic. After 15 days, both groups were subjected to a single session of 30 min of swimming with a load of 4.5% body weight attached to the dorsal region. Biochemical analyses were performed moments before, immediately after, and at 18 hrs, 24 hrs, 48 hrs, and 96 hrs after aerobic physical exercise through the collection of blood using heparinized capillary. The serum was withdrawn and used for determination of creatine kinase (CK). The CK concentrations were higher at the time immediately after exercise in diabetics (P < 0.05). In the control group, there was a tendency of decreased levels of CK after exercise, and at 24 hrs CK levels were lower than at pre exercise. There were significant differences between groups, thus characterizing a higher state of injury in diabetic animals throughout the experiment. Although moderate aerobic exercise caused a reduction of glucose levels in the diabetic rats, there was an increase in the levels of CK that reflects a greater muscular injury. Key Words: Aerobic Exercise, Creatine Kinase, Diabetes Mellitus