miR-138-5p targets sirtuini to regulate acute lung injury by regulation of the NF-[kappa]B signaling pathway

• Published in: Canadian journal of physiology and pharmacology Vol. 98; no. 8; pp. 522 - 530
• Main Authors: Wu, Yinshan, Jiang, Weiliang, Lu, Zhuhua, Su, Wei, Liu, Nan, Guo, Feng
• Format: Journal Article
• Language: English
• Published: NRC Research Press 01.08.2020
• Subjects: Health aspects; Lung diseases; Mitogens
• ISSN: 0008-4212; 1205-7541
• DOI: 10.1139/cjpp-2019-0559

Acute lung injury (ALI), a disease with a high mortality rate, is a noncardiogenic pulmonary inflammatory response and characterized by damage to the pulmonary system. In this study, we explored the mechanism of the occurrence and development of ALI. It was firstly found that miR-138-5p could inhibit the expression of sirtuin1 (SIRT1), and we further demonstrated that miR-138-5p targets directly SIRT1 through the luciferase assay, while the latter negatively regulated the expression of NF-[kappa]B. A549 cells were treated with lipopolysaccharide in vitro to simulate ALI cells and induce ALI in the model mice. The results showed that inhibiting the expression of miR-138-5p could effectively increase the viability of damaged cells, promote cell proliferation, reduce apoptosis, inhibit the inflammatory response, reduce oxidative stress, and then relieve ALI symptoms. Collectively, our results suggested that miR-138-5p can inhibit SIRT1 expression and indirectly activate the NF-[kappa]B signaling pathway, thus regulating the development of ALI.