Synthesis of Hybrid Gold Nanoparticle (AuNP) Functionalized Superparamagnetic Nanoparticles (SPMNPs) for Efficient Coupling of Biomolecules

Recently, we reported our methodology for isolating plasma membrane and lysosome from eukaryotic cell using superparamagnetic nanoparticles (SPMNPs). Here in this article, we report a step-by-step protocol for synthesis of hybrid gold nanoparticle (AuNP), surface functionalization of AuNPs on superp...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2125; p. 73
Main Authors Thimiri Govinda Raj, Deepak B, Khan, Niamat Ali, Venkatachalam, Srisaran, Arumugam, Sivakumar, Palaniswamy, Selvamani
Format Journal Article
LanguageEnglish
Published United States 2020
Subjects
Dynamic Light Scattering
Gold - chemistry
Magnetic Iron Oxide Nanoparticles - chemistry
Magnetic Iron Oxide Nanoparticles - ultrastructure
Metal Nanoparticles - chemistry
Phospholipids
SPMNPs
AuNPs
Bio-conjugation
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Summary:Recently, we reported our methodology for isolating plasma membrane and lysosome from eukaryotic cell using superparamagnetic nanoparticles (SPMNPs). Here in this article, we report a step-by-step protocol for synthesis of hybrid gold nanoparticle (AuNP), surface functionalization of AuNPs on superparamagnetic nanoparticles (SPMNPs), and potential use of hybrid AuNP-SPMNP for efficient coupling of biomolecules.
ISSN:1940-6029
DOI:10.1007/7651_2019_254