The Circadian Binding of CLOCK Protein to the Promoter of C/ebp[alpha] Gene in Mouse Cells

C/EBP[alpha] plays important roles in metabolism as well as in the maintenance of energy homeostasis. Here we describe loss of the circadian oscillation of C/ebp[alpha] expression in liver of Clock mutant mice. Reporter assays indicate Clock and Bmal significantly induced C/ebp[alpha] gene expressio...

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Published inPloS one Vol. 8; no. 3; p. e58221
Main Authors Kawasaki, Haruhisa, Doi, Ryosuke, Ito, Kumpei, Shimoda, Masami, Ishida, Norio
Format Journal Article
LanguageEnglish
Published Public Library of Science 11.03.2013
Subjects
Chromatin
Genes
Genetic aspects
House mouse
Protein binding
Transcription (Genetics)
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Summary:C/EBP[alpha] plays important roles in metabolism as well as in the maintenance of energy homeostasis. Here we describe loss of the circadian oscillation of C/ebp[alpha] expression in liver of Clock mutant mice. Reporter assays indicate Clock and Bmal significantly induced C/ebp[alpha] gene expression whereas Cry suppressed. Real time reporter assays showed that two mutated E-boxes disrupted C/ebp[alpha] promoter dependent-oscillation. Chromatin immunoprecipitation suggests Clock can bind to two E-boxes in the C/ebp[alpha] promoter with a circadian manner in vivo. Thus, C/ebp[alpha] gene transcription is under circadian control of a core clock component, Clock. The data suggests that circadian disturbances may affect metabolic abnormalities through the C/ebp[alpha] pathway in liver.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0058221