Phage Nanobioparticle Expressing Apoptin Efficiently Suppress Human Breast Carcinoma Tumor Growth In Vivo

• Published in: PloS one Vol. 8; no. 11; p. e79907
• Main Authors: Shoae-Hassani, Alireza, Keyhanvar, Peyman, Seifalian, Alexander Marcus, Mortazavi-Tabatabaei, Seyed Abdolreza, Ghaderi, Narmin, Issazadeh, Khosro, Amirmozafari, Nour, Verdi, Javad
• Format: Journal Article
• Language: English
• Published: Public Library of Science 22.11.2013
• Subjects: Apoptosis; Breast cancer; Cancer treatment; Carcinoma; Growth; Health aspects
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0079907

Using phages is a novel field of cancer therapy and phage nanobioparticles (NBPs) such as [lambda] phage could be modified to deliver and express genetic cassettes into eukaryotic cells safely in contrast with animal viruses. Apoptin, a protein from chicken anemia virus (CAV) has the ability to specifically induce apoptosis only in carcinoma cells. We presented a safe method of breast tumor therapy via the apoptin expressing [lambda] NBPs. Here, we constructed a [lambda] ZAP-CMV-apoptin recombinant NBP and investigated the effectiveness of its apoptotic activity on BT-474, MDA-MB-361, SKBR-3, UACC-812 and ZR-75 cell lines that over-expressing her-2 marker. Apoptosis was evaluated via annexin-V fluorescent iso-thiocyanate/propidium iodide staining, flow-cytometric method and TUNEL assay. Transfection with NBPs carrying [lambda] ZAP-CMV-apoptin significantly inhibited growth of all the breast carcinoma cell lines in vitro. Also nude mice model implanted BT-474 human breast tumor was successfully responded to the systemic and local injection of untargeted recombinant [lambda] NBPs. The results presented here reveal important features of recombinant [lambda] nanobioparticles to serve as safe delivery and expression platform for human cancer therapy.