Two Wheat Glutathione Peroxidase Genes Whose Products Are Located in Chloroplasts Improve Salt and H.sub.2O.sub.2 Tolerances in Arabidopsis

Oxidative stress caused by accumulation of reactive oxygen species (ROS) is capable of damaging effects on numerous cellular components. Glutathione peroxidases (GPXs, EC 1.11.1.9) are key enzymes of the antioxidant network in plants. In this study, W69 and W106, two putative GPX genes, were obtaine...

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Published inPloS one Vol. 8; no. 10; p. e73989
Main Authors Zhai, Chao-Zeng, Zhao, Lei, Yin, Li-Juan, Chen, Ming, Wang, Qing-Yu, Li, Lian-Cheng, Xu, Zhao-Shi, Ma, You-Zhi
Format Journal Article
LanguageEnglish
Published Public Library of Science 02.10.2013
Subjects
Arabidopsis thaliana
Genetic aspects
Peroxidase
Physiological aspects
Wheat
China
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Summary:Oxidative stress caused by accumulation of reactive oxygen species (ROS) is capable of damaging effects on numerous cellular components. Glutathione peroxidases (GPXs, EC 1.11.1.9) are key enzymes of the antioxidant network in plants. In this study, W69 and W106, two putative GPX genes, were obtained by de novo transcriptome sequencing of salt-treated wheat (Triticum aestivum) seedlings. The purified His-tag fusion proteins of W69 and W106 reduced H.sub.2 O.sub.2 and t-butyl hydroperoxide (t-BHP) using glutathione (GSH) or thioredoxin (Trx) as an electron donor in vitro, showing their peroxidase activity toward H.sub.2 O.sub.2 and toxic organic hydroperoxide. GFP fluorescence assays revealed that W69 and W106 are localized in chloroplasts. Quantitative real-time PCR (Q-RT-PCR) analysis showed that two GPXs were differentially responsive to salt, drought, H.sub.2 O.sub.2, or ABA. Isolation of the W69 and W106 promoters revealed some cis-acting elements responding to abiotic stresses. Overexpression of W69 and W106 conferred strong tolerance to salt, H.sub.2 O.sub.2, and ABA treatment in Arabidopsis. Moreover, the expression levels of key regulator genes (SOS1, RbohD and ABI1/ABI2) involved in salt, H.sub.2 O.sub.2 and ABA signaling were altered in the transgenic plants. These findings suggest that W69 and W106 not only act as scavengers of H.sub.2 O.sub.2 in controlling abiotic stress responses, but also play important roles in salt and ABA signaling.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0073989