Preliminary analysis of single-nucleotide polymorphisms in IL-10, IL-4, and IL-4R[alpha] genes and profile of circulating cytokines in patients with gastric Cancer

• Published in: BMC gastroenterology Vol. 18; no. 1
• Main Authors: Cárdenas, Denny Miley, Sánchez, Angie Carolina, Rosas, Daris Angélica, Rivero, Esmeralda, Paparoni, Massiel Dayana, Cruz, Mildred Andreína, Suárez, Yeicy Paola, Galvis, Nestor Fabián
• Format: Journal Article
• Language: English
• Published: BioMed Central Ltd 10.12.2018
• Subjects: Cytokines; Genetic aspects; Genetic polymorphisms; Interleukin-10; Interleukin-4; Single nucleotide polymorphisms; Stomach cancer; Colombia
• ISSN: 1471-230X; 1471-230X
• DOI: 10.1186/s12876-018-0913-9

Background Gastric Cancer is highly prevalent and deadly worldwide. In Colombia, it is the most lethal form of cancer. Some single-nucleotide polymorphisms in IL-10, IL-4, and IL-4R[alpha] genes have been associated with an anti-inflammatory environment and a Th2 profile in detriment of the antitumor Th1 response. This research sought to detect single-nucleotide polymorphisms in promoter sequences, like - 1082 (G/A), - 592 (C/A), and - 819 (C/T), as well as - 590 (C/T) of the IL-10 and IL-4 genes, respectively; in addition to the IL-4R[alpha] mutation variants, Ile50Val and Q576R, together with circulating levels of IL-4, TNF-[alpha], IL-10, and IFN-[gamma] in patients with gastric carcinoma in Cúcuta, Colombia. Methods In a cross-sectional study, 17 patients and 30 healthy individuals were genotyped for the six polymorphisms mentioned through PCR-RFLP of DNA obtained from peripheral blood cells and serum samples were analyzed by sandwich ELISA to quantify cytokines. Statistical difference between groups was determined along with the association between the presence of polymorphisms and the risk of gastric cancer, as well as the mortality in patients, using Mann-Whitney U test and logistic regression analysis, respectively. Results An association between the - 1082 (G/A) and the risk of gastric cancer was found (OR = 7.58, range 0.77-74.06, P = 0.08). Furthermore, patients had a significant increase in IL-4 serum levels (P < 0.01) compared to healthy individuals, both variables showed a higher estimated risk of mortality in patients, although without statistical association (P > 0.05). Conclusion We infer that two possible biomarkers (one immunological and one genetic) could be considered in association with gastric cancer in our population, which should be confirmed by subsequent studies involving a greater number of individuals. Keywords: Genetic polymorphism, Cytokines, Neoplasia