Thermostable lipase production by Aneurinibacillus thermoaerophilus MBW2 strain isolated from Indian hot water spring

Four hot water springs viz., Manikaran, Vashisht, Khirganga and Tattapani were purposely selected for isolation of thermostable lipase producing bacterial isolates. The pH and temperature of the four thermal springs were ranged from 4.0-6.0 and 51105[degrees]C respectively. Isolated forty two thermo...

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Published inJournal of pure & applied microbiology : an international research journal of microbiology Vol. 10; no. 2; p. 1197
Main Authors Sharma, Ankita, Dhiman, Karuna, Ansari, Zeba, Shirkot, Poonam
Format Journal Article
LanguageEnglish
Published Oriental Scientific Publishing Company 01.06.2016
Subjects
Bacillus
Health aspects
Lipase
Physiological aspects
Springs (Water)
India
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Summary:Four hot water springs viz., Manikaran, Vashisht, Khirganga and Tattapani were purposely selected for isolation of thermostable lipase producing bacterial isolates. The pH and temperature of the four thermal springs were ranged from 4.0-6.0 and 51105[degrees]C respectively. Isolated forty two thermophilic bacterial isolates, were described as putative thermostable lipase producers on the basis of their ability to form zone of clearance on tributyrin agar medium. Quantitative screening led to the selection of MBW2 bacterial isolate showing maximum thermostable lipase activity of 4.83U/ml after 24 hrs of incubation time at 60[degrees] C temperature, was selected for morphological, biochemical and molecular characterization. Genomic DNA isolated from the selected MBW2 bacterial isolate was subjected to PCR amplification followed by sequencing using universal primers for 16S rrna gene. In silico molecular analysis identified MBW2 bacterial isolate as Aneurinibacillus thermoaerophilus strain MBW2. Optimum culture conditions for growth and thermostable lipase activity of selected isolate were used for the enzyme production which was purified to 3.08 fold with percent yield of 13.73% using ammonium sulphate precipitation technique, gel filteration chromatography and ion exchange chromatography technique. The molecular weight of the purified enzyme was found to be 42.5 kDa using SDS-PAGE. Keywords: Thermostable lipase, thermophiles, hot water spring.
ISSN:0973-7510