Antithrombotic and antiinflammatory effect of heparin conjugated poly on inflammation induced cyclooxygenase

Living tissues elicits a strong inflammatory response, once it comes in contact with artificial surface; including activation of various blood components. This study provides a brief background of anti-thrombotic and anti-inflammatory properties of heparinized polymer (H-PLLA) compared to non-hepari...

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Bibliographic Details
Published inTrends in biomaterials & artificial organs Vol. 30; no. 2; p. 80
Main Authors Mandal, Ramila D, Patel, Payal J, Patel, Nisha R, Patel, Himanshu J, Kale, Sunil P, Engineer, Chhaya B, Raval, Ankur J
Format Journal Article
LanguageEnglish
Published Society for Biomaterials and Artificial Organs 01.04.2016
Subjects
Anti-inflammatory agents
COX-2 inhibitors
Dosage and administration
Fibrinolytic agents
Health aspects
Heparin
Inflammation
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Summary:Living tissues elicits a strong inflammatory response, once it comes in contact with artificial surface; including activation of various blood components. This study provides a brief background of anti-thrombotic and anti-inflammatory properties of heparinized polymer (H-PLLA) compared to non-heparinized polymer in vitro. The anti-thrombotic and anti-inflammatory potential was assessed through effect on enzymatic activity of Human Platelet Cyclooxygenase 1 (COX-1) and Human Monocyte Cyclooxygenase 2 (COX-2) respectively, on exposure of heparin-conjugated polymer (CP) and heparin non-conjugated polymer (NCP) to human blood. The enzymatic activity of COX-1 and COX-2 was determined by measuring the concentration of their specific products, i.e. Thromboxane B2 (TXB2) and Prostaglandin E2 (PGE2), respectively. Test item was exposed to non-heparinized human blood. The TXB2 serum level and PGE2 plasma level were determined using enzyme immunoassay (EIA). No significant dose-responsive change was observed in COX-1 activity for both the types of test materials. A significant decrease in COX-2 activity was observed with increasing polymer concentrations in case of Heparinized Poly-L-Lactide (H-PLLA) compared to non-heparinized polymer.
ISSN:0971-1198