CK1[alpha] overexpression correlates with poor survival in colorectal cancer

Background Colorectal cancer (CRC) is the fourth leading cause of cancer related deaths worldwide and prognosis in advanced tumor stage still remains poor. Since CK1 isoforms have been reported to be deregulated in several tumor entities CK1 has emerged as a novel drug target in cancer therapy. In t...

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Bibliographic Details
Published inBMC cancer Vol. 18; no. 1
Main Authors Richter, Julia, Kretz, Anna-Laura, Lemke, Johannes, Fauler, Michael, Werner, Jens-Uwe, Paschke, Stephan, Leithäuser, Frank, Henne-Bruns, Doris, Hillenbrand, Andreas, Knippschild, Uwe
Format Journal Article
LanguageEnglish
Published BioMed Central Ltd 06.02.2018
Subjects
Adjuvant chemotherapy
Biological markers
Colorectal cancer
Mutation
Prognosis
Risk factors
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Summary:Background Colorectal cancer (CRC) is the fourth leading cause of cancer related deaths worldwide and prognosis in advanced tumor stage still remains poor. Since CK1 isoforms have been reported to be deregulated in several tumor entities CK1 has emerged as a novel drug target in cancer therapy. In this study we set out to investigate whether CK1[alpha] might have the potential to serve as prognostic marker. Methods CK1[alpha] RNA and protein expression levels in healthy and tumor tissue of CRC patients were analyzed using quantitative real-time PCR and Western Blot analysis, respectively. Prognostic relevance was investigated by correlating obtained CK1[alpha] expression levels with patients' survival rate generating Kaplan-Meier survival plots. Results It could be shown that CK1[alpha] is overexpressed in colorectal tumor tissue compared to normal tissue and CK1[alpha] overexpression in tumor tissue correlates with poor survival in CRC patients. Results become more significant when only considering patients with high-grade tumors, as well as patients assigned to UICC II and UICC III stage. Furthermore, Cox regression analysis revealed that CK1[alpha] is an independent prognostic factor. In addition, tumors expressing decreased levels of the kinase reveal positive effects on overall survival when localized in the right colon compared to those in the left side. Conclusion In summary, this study provides evidence for the first time that CK1[alpha] RNA levels might serve as prognostic marker for CRC. Keywords: Casein kinase 1 alpha, Biomarker, Colorectal cancer (CRC), Survival, Prognosis, Drug target
ISSN:1471-2407
1471-2407
DOI:10.1186/s12885-018-4019-0