Anatomy and micromorphometric analysis of leaf Catasetum x apolloi benelli grade with addition of potassium silicate under different light sources/Anatomia e analise micromorfometrica foliar de Catasetum x apolloi benelli grade com adicao de silicato de potassio em diferentes fontes de luz

• Published in: Brazilian journal of biology Vol. 77; no. 1; pp. 140 - 149
• Main Authors: Eburneo, L, Ribeiro-Junior, N.G, Karsburg, I.V, Rossi, A.A.B, Silva, I.V
• Format: Journal Article
• Language: English
• Published: São Carlos Association of the Brazilian Journal of Biology 01.01.2017; Instituto Internacional de Ecologia
• Subjects: Acclimatization; Agar; Anatomy; Autoclaving; Botany; Cell culture; Cell division; Differentiation; Elongation; Guard cells; Hypodermis; In vitro methods and tests; Light sources; Luminous intensity; Mesophyll; pH effects; Plant sciences; Potassium; Rib; Seeds; Stomata; Veins (plant anatomy); Brasil; Brazil
• ISSN: 1519-6984; 1678-4375
• DOI: 10.1590/1519-6984.12015

The aim of this study was to compare vitroplants Catasetum x apolloi grown under natural light and artificial light and different concentrations of potassium silicate, providing data on the anatomical differentiation that aids the acclimatization process of this species. Plants from in vitro seeding were used; 5 protocorms of approximately 0.5 cm were inoculated into vials with a capacity of 500 mL containing 100 mL of alternative culture medium plus potassium silicate (0.0, 0.5; 1.0 mL [L.sup.-1])pH adjusted to 5.5 [+ or -] 0.5 and gelated with 4G[L.sup.-1] agar before the autoclaving process. Cultures were maintained under natural light (TNE) and artificial light (TAE) for 90 days, and micromorphometric analysis was performed for polar and equatorial diameter, density and stomatal index, blade thickness in the central rib, and secondary veins. Applications in [K.sub.2]Si[O.sub.4] alternative medium provided the following: elongation of the hypodermis, thicker mesophyll, and more prominent midrib; elipptical guard cells; formation of epistomatal chamber; and lower stomatal density and stomatal with lower equatorial and polar diameters. The conditions that favored the acclimatization were lower light intensities and lower potassium silicate doses.