Effect of 17-[alpha] hydroxyprogesterone caproate on the production of tumor necrosis factor-[alpha] and the expression of cyclooxygenase-2 in lipopolysaccharide-treated gravid human myometrial explants

Objective: To determine whether 17-[alpha] hydroxyprogesterone (17-OHPC) alters tumor necrosis factor-[alpha] (FTNF-[alpha]) production and the expression of cyclooxygenase type 2 (COX-2) in myometrium exposed to lipopolysaccharide (LPS). Study Design: Lower segment myometrial biopsies were obtained...

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Bibliographic Details
Published inJournal of perinatology Vol. 30; no. 9; p. 584
Main Authors Patel, S, Li, A, Goodwin, T.M, Brower, M, Blitz, M, Minoo, P, Felix, J.C, Lee, R.H
Format Journal Article
LanguageEnglish
Published New York Nature Publishing Group 01.09.2010
Subjects
Biopsy
Cyclooxygenases
Cytokines
Enzymes
Gynecology
Health aspects
Hydroxyprogesterone
Infections
Myometrium
Obstetrics
Physiological aspects
Pilot projects
Pregnancy
Premature birth
Tumor necrosis factor
Tumor necrosis factor-TNF
United States
Los Angeles California
Southern California
United States > US
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Summary:Objective: To determine whether 17-[alpha] hydroxyprogesterone (17-OHPC) alters tumor necrosis factor-[alpha] (FTNF-[alpha]) production and the expression of cyclooxygenase type 2 (COX-2) in myometrium exposed to lipopolysaccharide (LPS). Study Design: Lower segment myometrial biopsies were obtained from non-laboring patients at term. Tissues were cultured in serum-free media with 17-OHPC (1 [micro]m) and LPS (1 mg/ml), either alone or in combination. At 24 h, the production of tumor necrosis factor-[alpha] (FTNF-[alpha]) and the expression of COX-2 was determined using enzyme linked immunosorbent assay and real-time (RT-PCR). Statistical analysis was performed using non-parametric testing. A P-value of <0.05 was considered significant. Result: 17-OHPC had no effect on FTNF-[alpha] production and COX-2 expression when compared with untreated myometrial explants (P = 0.61 and P = 0.95). LPS induced production of FTNF-[alpha] (P = 0.03) and expression of COX-2 (P = 0.02). Treatment with 17-OHPC did not block LPS-induced FTNF-[alpha] production (P = 0.37) or COX-2 expression (P = 0.12). Conclusion: In this pilot study, 17-OHPC did not affect the production of FTNF-[alpha] or COX-2 expression in human myometrium. Journal of Perinatology (2010) 30, 584-589; doi: 10.1038/jp.2010.23; published online 11 March 2010 Keywords: 17-[alpha] hydroxyprogesterone; caproate; cyclooxygenase-2; tumor necrosis factor; pregnancy; myometrium; human
ISSN:0743-8346
1476-5543
DOI:10.1038/jp.2010.23