Impairment of tissue repair in pneumonia due to [beta]-cell deficiency: role of endoplasmic reticulum stress in alveolar macrophages

Diabetes mellitus (DM) patients are susceptible to delayed resolution of pneumonia. However, the pathogenesis of the impaired tissue repair in inflamed lungs in diabetic patients is unknown. We evaluated phagocytosis of apoptotic cells (efferocytosis), hepatocyte growth factor (HGF) production in br...

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Bibliographic Details
Published inBMC research notes Vol. 12; no. 1
Main Authors Yamashita, Yoshiro, Kuroki, Reiki, Takaki, Masahiro, Tanaka, Takeshi, Senba, Masachika, Morimoto, Konosuke, Amano, Hideaki
Format Journal Article
LanguageEnglish
Published BioMed Central Ltd 22.03.2019
Subjects
Apoptosis
Diabetics
DNA
DNA synthesis
Epithelium
Immunohistochemistry
Lung diseases
Macrophages
Pancreatic beta cells
Pneumonia
Streptozocin
Stress (Physiology)
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Summary:Diabetes mellitus (DM) patients are susceptible to delayed resolution of pneumonia. However, the pathogenesis of the impaired tissue repair in inflamed lungs in diabetic patients is unknown. We evaluated phagocytosis of apoptotic cells (efferocytosis), hepatocyte growth factor (HGF) production in bronchoalveolar lavage fluid (BALF), and lung histology in the resolution phase following acute lung injury in streptozotocin (STZ)-induced [beta]-cell-depleted hyperglycemic mice. We also investigated efferocytosis and HGF production by macrophages under [beta]-cell depletion condition ex vivo. In [beta]-cell-depleted mice, efferocytosis was not significantly different from that in control mice; however, the concentration of HGF in BALF was decreased. In addition, diminished HGF production by alveolar macrophages and DNA synthesis in the alveolar epithelium was observed by immunohistochemistry. Ex vivo experiments confirmed that HGF production by macrophages was impaired under [beta]-cell depletion probably because of endoplasmic reticulum stress.
ISSN:1756-0500
1756-0500
DOI:10.1186/s13104-019-4209-0