Optimization of differentiation protocols of dental tissues stem cells to pancreatic [beta]-cells

Despite the recent progress in the differentiation strategies of stem cells into pancreatic beta cell lineage, current protocols are not optimized for different cell types. The purpose of this study is to investigate and compare the ability of stem cells derived from dental pulp (DPSCs) and periodon...

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Bibliographic Details
Published inBMC molecular and cell biology Vol. 23; no. 1
Main Authors Aly, Riham M, Aglan, Hadeer A, Eldeen, Ghada Nour, Ahmed, Hanaa H
Format Journal Article
LanguageEnglish
Published BioMed Central Ltd 20.09.2022
Subjects
Analysis
Cell differentiation
Complications and side effects
Dentistry
Diabetes
Methods
Pancreatic beta cells
Services
Egypt
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Summary:Despite the recent progress in the differentiation strategies of stem cells into pancreatic beta cell lineage, current protocols are not optimized for different cell types. The purpose of this study is to investigate and compare the ability of stem cells derived from dental pulp (DPSCs) and periodontal ligament (PDLSCs) as two anatomically different dental tissues to differentiate into pancreatic beta cells while assessing the most suitable protocol for each cell type. DPSCs & PDLSCs were isolated and characterized morphologically and phenotypically and then differentiated into pancreatic beta cells using two protocols. Differentiated cells were assessed by qRT-PCR for the expression of pancreatic related markers Foxa-2, Sox-17, PDX-1, Ngn-3, INS and Gcg. Functional assessment of differentiation was performed by quantification of Insulin release via ELISA. Protocol 2 implementing Geltrex significantly enhanced the expression levels of all tested genes both in DPSCs & PDLSCs. Both DPSCs & PDLSCs illustrated improved response to increased glucose concentration in comparison to undifferentiated cells. Moreover, DPSCs demonstrated an advanced potency towards pancreatic lineage differentiation over PDLSCs under both protocols. In conclusion, the current study reports the promising potential of dental derived stem cells in differentiating into pancreatic lineage through selection of the right protocol.
ISSN:2661-8850
2661-8850
DOI:10.1186/s12860-022-00441-6