Combination of serum protein electrophoresis, serum immunofixation electrophoresis and serum free light chain assay in the diagnosis of monoclonal gammopathies

Introduction: Demonstration of monoclonal immunoglobulin molecule in serum and/or urine forms the mainstay in the diagnosis of monoclonal gammopathies (MG's). The major tests that help in this regard are Serum Protein Electrophoresis (SPEP), serum Immunofixation Electrophoresis (sIFE) and serum...

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Bibliographic Details
Published inIndian journal of clinical biochemistry Vol. 31; no. S1; p. S69
Main Authors Kuriakose, Eldho, Subhakumari, K.N, Unni C., Sumithra N, Vasudevan, Anu
Format Journal Article
LanguageEnglish
Published Springer 01.12.2016
Subjects
Blood proteins
Electrophoresis
Medical research
Medicine, Experimental
Multiple myeloma
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Summary:Introduction: Demonstration of monoclonal immunoglobulin molecule in serum and/or urine forms the mainstay in the diagnosis of monoclonal gammopathies (MG's). The major tests that help in this regard are Serum Protein Electrophoresis (SPEP), serum Immunofixation Electrophoresis (sIFE) and serum Free Light Chain assay (sFLC). Aim: To study the combination of SPEP, sIFE and sFLC in the diagnosis of monoclonal gammopathies. Materials and Methods: The study was conducted over a period of 1 year from May 2015 to May 2016, amongst the patients attending the outpatient and inpatient wings of medical oncology department of Amrita Institute of Medical Sciences and Research Centre, Kochi. The study consisted of 30 patients diagnosed with monoclonal gammopathy (mainly multiple myeloma). Results: Out of the 30 patients diagnosed with a monoclonal gammopathy (mainly myeloma in this study)--SPE alone could detect only 21 cases (70%). A combination of SPE + sFLC could detect 28 cases (93.33%) and a combination of SPE + sIFE could detect 29 cases (96.67%). Whereas, a combination of all three tests could detect all 30 cases of myeloma. (100%). Conclusion: A combination panel of these 3 tests prove to be more useful than individual tests for the detection of myeloma.
ISSN:0970-1915
0974-0422