EVALUATION OF IMMUNOMODULATORY POTENTIAL OF FILARIAL ANTIGENS ON THE DEVELOPMENT OF TYPE 1 DIABETES

Background: In recent years substantial epidemiological and experimental evidence became available to support the hypothesis that infection with helminths can suppress the development of type 1 diabetes. Understanding modulation of the host immune system by pathogens offers rich protective approach....

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Bibliographic Details
Published inIndian journal of clinical biochemistry Vol. 27; no. S1; p. S62
Main Authors Hivre, Manjusha, Khan, Mohammad Sameer, M.H, Amdare, Nitin, Yadav, Ravi, Khatri, Vishal, Reddy, M.V.R
Format Journal Article
LanguageEnglish
Published Springer 24.05.2022
Subjects
Analysis
Animal experimentation
Antigens
Blood sugar
Epidemiology
Health aspects
Pancreatic beta cells
Prevention
Proteins
Type 1 diabetes
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Summary:Background: In recent years substantial epidemiological and experimental evidence became available to support the hypothesis that infection with helminths can suppress the development of type 1 diabetes. Understanding modulation of the host immune system by pathogens offers rich protective approach. Hence, identification and characterization of helminths derived immunomodulatory molecules that contribute to the protective effect could lead to new protective approaches in type 1 diabetes. Objective: To evaluate the immunomodulatory and prophylactic effect of filarial immune modulators (Bm mf's/ Bmmf'ES'/ rBmCys) on the development of type 1 diabetes. Methodology: BALB/c mice (n=7-8) were pretreated with 4 doses of filarial derived proteins (Bm mf's/ Bmmf'ES/ rBmCys) in alum adjuvant (25pg) for 2 months (at intervals of 15 days) followed by the induction of diabetes using streptozotocin (STZ). The mice was followed up by monitoring their plasma fasting glucose levels. Results: At the end of experiment period, the group of mice (n=7) pre-treated with rBmCys followed by induction of diabetes (rBmCys-STZ) showed significantly lower fasting blood glucose level compared to the levels in mice (n=7) pre-treated with only alum adjuvant (Alum--STZ) (p<0.01). Also there was significant reduction in the blood glucose levels in the mice pretreated with Bm mf's (n=7, p<0.001) rBmmf'ES (n=7, p<0.001). Consistent with these changes in glucose levels, mice pre-treated with filarial proteins showed almost normal pancreatic islet cell architecture with minimal inflammatory change. Conclusion: Present study provided evidence of exploiting filarial derived proteins for the prevention against type 1 diabetes through immune modulation.
ISSN:0970-1915
0974-0422