Hepatoprotective Potential of Tridax Procumbens Against Isoniazid And Rifampicin-Induced Hepatotoxicity In Albino Rats

• Published in: Indian journal of clinical biochemistry Vol. 32; no. S1; p. S213
• Main Authors: Sagheer, Rida, Singh, R, Nasibullah, M, Sibghatullah, M, Shukla. M, Firoj, H, Mahdi, A.A, Khan, A.R
• Format: Journal Article
• Language: English
• Published: Springer 24.05.2022
• Subjects: Antioxidants; Isoniazid; Liver; Rifampin; Superoxide; Tuberculosis
• ISSN: 0970-1915; 0974-0422

BACKGROUND AND OBJECTIVE: Tridax procumbens belongs to Asteraceae family has been demonstrated to be hepatoprotective by Ayurvedic practitioner but it has not been scientifically explored so for. Therefore the present study was carried out for the assessment of hepatoprotective role of T. procumbens against anti-tuberculosis drug--induced hepatotoxicity in male Wistar rat. MATERIALS AND METHODS: Male Wistar albino rats weighing 100-150g divided into 7 groups of 6 animals each. Group-1 (control) normal saline 1ml/Kg p.o. Group-2 (positive) silymarine- suspension 200 mg/ Kg p. o. for 14 days. Group-3 (toxicant)--INH (Isoniazid) & RMP (Rifampicin) (50mg/Kg, 100mg/kg) i. p. on day 14th. Group-4 silymarine same as group 2 for 14 days and toxin on day 14th Group-5 (treatment) MTPL (methanolic extracts of T.procumbens leaves) 300mg/ Kg p. o. for 14 days and toxin as same dose in group 4 Group-6 MTPS (methanolic extracts of T. procumbens stem) same as group 5 Group-7 MTPF (methanolic extracts of T. procumbens flower) same as in group 5. Blood was collected from orbital plexus and liver was dissected out. LFT, Creatinine and cholesterol were measured and liver was subjected for histopathological examination. The consequences of MTPL, MTPS and MTPF on antioxidant enzymes SOD (superoxide dismutase) and CAT (catalase) were monitored to assess antioxidant activity. RESULTS: SGOT, SGPT ALP and total bilirubin were significantly elevated in INH & RMP treated group and displayed considerable decrease in SOD, CAT and TP in a dose dependent manner. Whereas MTPL (300mg/ kg p. o.) showed more significant reduction of in these parameters and increasing the SOD, CAT and TP levels. MTPL was found to have hepatoprotective potential as compare to MTPS and MTPF. CONCLUSION: It may be due to numerous reasons like antioxidant and free radicals scavenge property, ability to stabilizing the hepatic cells and reducing neutrophil infiltration into the hepatocytes. KEY WORDS: MTPL, isoniazid, rifampicin, SOD, CAT, total protein, hepatoprotection