Exogenously Applied Cytokinin Altered the Bacterial Release and Subsequent Stages of Nodule Development in Pea IIpd3/Cyclops/I Mutant

Regulation of plant hormonal status is one of the major targets of symbiotic signaling during nodule formation in legume plants. However, the genetic and hormonal networks that regulate transition to differentiation of nodules are not well-characterized in legume plants. Analysis of plant mutants fo...

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Bibliographic Details
Published inPlants (Basel) Vol. 12; no. 3
Main Authors Kantsurova (Rudaya), Elizaveta S, Ivanova, Alexandra N, Kozyulina, Polina Y, Dolgikh, Elena A
Format Journal Article
LanguageEnglish
Published MDPI AG 01.02.2023
Subjects
Analysis
Beans
Botanical research
Cytokinins
Gene mutations
Genetic aspects
Genetic transcription
Health aspects
Infection
Legumes
Mimosaceae
Plant hormones
Russia
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Summary:Regulation of plant hormonal status is one of the major targets of symbiotic signaling during nodule formation in legume plants. However, the genetic and hormonal networks that regulate transition to differentiation of nodules are not well-characterized in legume plants. Analysis of plant mutants forming nodules impaired in rhizobial infection allowed us to identify some regulators involved in the control of the later stages of nodule development. In the current work, we extend our earlier studies on the influence of exogenously applied cytokinin on the later stages of nodule morphogenesis using pea sym33 (ipd3/cyclops) mutants impaired in the gene encoding IPD3/CYCLOPS transcription factor. One of the noticeable effects of the influence of exogenously applied cytokinin on nodules in the sym33-3 mutant was an increasing size of these structures. Cytokinin treatment was shown to stimulate bacterial release and increase the percentage of infected cells in nodules. To explore the role of possible regulators of nodule differentiation, we performed searching in pea transcriptome. The transcriptome study in pea P. sativum revealed the importance of the CCS52 regulator, EFD transcription factor, SYMREM regulator, RSD, the MADS-domain/AGL, and SHORT INTERNODE/STYLISH gene families encoding transcription factors in the control of nodule differentiation. Analysis of the expression patterns was verified by real-time PCR in response to exogenously applied cytokinin treatment.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants12030657