Effect of Eupatorium japonicum Extract on the Metastasis, Invasion and Adhesion of MDA-MB-231 Human Breast Cancer Cells

• Published in: Korean Journal of Food Science and Technology Vol. 43; no. 2
• Main Authors: Woo, E.Y., Hallym University, Chuncheon, Republic of Korea, Park, S.Y., Hallym University, Chuncheon, Republic of Korea, Kwon, S.J., Hallym University, Chuncheon, Republic of Korea, Kwon, G.T., Hallym University, Chuncheon, Republic of Korea, Kim, J.D., Kangwon National University, Chuncheon, Republic of Korea, Lim, S.S., Hallym University, Chuncheon, Republic of Korea, Yoon, J.H., Hallym University, Chuncheon, Republic of Korea
• Format: Journal Article
• Language: Korean
• Published: 01.04.2011
• Subjects: breast cancer; CELLS; CELLULE; CELULAS; Eupatorium japonicum; matrix metalloproteinase; metastasis; urokinase-type plasminogen activator
• ISSN: 0367-6293
• DOI: 10.9721/KJFST.2011.43.2.213

The metastatic effect of Eupatorium japonicum extract (EJE) on MDA-MB-231 human breast cancer cells was investigated. MDA-MB-231 cells were treated with various concentrations of EJE (0, 5, 10 and 20 ㎍/mL). EJE inhibited cell migration, invasion and adhesion of MDA-MB-231 cells in dose-dependent manners. Gelatin zymography exhibited that EJE significantly down regulated secretion of matrix metalloproteinase (MMP)-9 and MMP-2. EJE decreased the protein levels of tissue inhibitor of metalloproteinase (TIMP)-1 but increased TIMP-2 levels. Additionally, EJE reduced the protein and mRNA levels of urokinase-type plasminogen activator (uPA), vascular endothelial growth factor (VEGF) and intercellular adhesion molecule (ICAM). In several solvent fractions of EJE, the hexane fraction markedly decreased MDAMB-231 cell migration. Thus, these finding suggest that EJE may be a potential antimetastatic agent, which can considerably inhibit the metastatic and invasive capacity of breast cancer cells.