Preparation of Hot Water Extracts of Dandelion Leaves to Increase Anti-inflammatory Activity
To materialize anti-inflammatory compounds from dandelion leaves, we studied optimal conditions of hot water extraction and concentration. We investigated the properties of extracts on various temperatures between 60 and 90℃. Extraction yields at 60℃ were the highest at 39.2±1.3% and flavonoid conte...
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Published in | Han'guk Sikp'um Yŏngyang Kwahakhoe chi Vol. 38; no. 3 |
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Main Authors | , , , , |
Format | Journal Article |
Language | Korean |
Published |
01.03.2009
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Subjects | |
Online Access | Get more information |
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Summary: | To materialize anti-inflammatory compounds from dandelion leaves, we studied optimal conditions of hot water extraction and concentration. We investigated the properties of extracts on various temperatures between 60 and 90℃. Extraction yields at 60℃ were the highest at 39.2±1.3% and flavonoid contents were increased by heating. However, anti-inflammatory activities were remarkably decreased by increasing temperature. According to the results that the highest anti-inflammatory activities of water extract were demonstrated at 60℃, cell wall degrading enzyme treatment was carried out to increase extraction yield of anti-inflammatory compounds. The enzyme treatment resulted in increased yields and flavonoids from dandelion leaves of 1.5-fold and anti-inflammatory activities were not decreased. Also, column chromatography was used for concentration of anti-inflammatory compounds from extract of dandelion leaves treated with enzyme. Anti-inflammatory activities of adsorbed compounds on Amberlite XAD-2 and Sepabeads SP-850 were significantly increased. However, yields of adsorbed compounds were increased over two-folds on Sepabeads SP-850 compared to Amberlite XAD-2. Thus Sepabeads SP-850 was more effective than Amberlite XAD-2 to concentrate the anti-inflammatory compounds of dandelion leaves. |
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Bibliography: | 2010002009 Q01 |
ISSN: | 1226-3311 |
DOI: | 10.3746/jkfn.2009.38.3.391 |