Carbohydrase production systems from ectomycorrhizal fungi, Tricholoma matsutake and Lyophyllum shimeji

• Published in: Transactions of the Mycological Society of Japan Vol. 50; no. 2
• Main Author: Kusuda, M., Osaka Prefecture Univ., Sakai (Japan)
• Format: Journal Article
• Language: Japanese
• Published: 01.11.2009
• Subjects: CHAMPIGNON COMESTIBLE; CULTURAL METHODS; EDIBLE FUNGI; ESTERASAS; ESTERASE; ESTERASES; ESTUDIOS DE FACTIBILIDAD; ETUDE DE FAISABILITE; FEASIBILITY STUDIES; http://www.fao.org/aos/agrovoc#c_2487; http://www.fao.org/aos/agrovoc#c_2670; http://www.fao.org/aos/agrovoc#c_26781; http://www.fao.org/aos/agrovoc#c_29762; http://www.fao.org/aos/agrovoc#c_31711; http://www.fao.org/aos/agrovoc#c_4273; LEPISTA; LYOPHYLLUM; METODOS DE CULTIVO; MODE DE CULTURE; SETAS COMESTIBLES
• ISSN: 0029-0289
• DOI: 10.18962/jjom.jjom.H20-07

Ohta (1994) reported that Lyophyllum shimeji classified as an ectomycorrhizal fungus similar to Tricholoma matsutake forms mature fruit-bodies without a host plant in a bottle containing a barley grain cultivation medium. Therefore, the amylase production systems of L. shimeji based on the utilization of barley starch as a growth substrate were examined. Results showed for the first time that the purified amylase is a glucoamylase that has a high hydrolyzing potential. From this point of view, the saccharide substrate hydrolyzing enzymes of T. matsutake were also investigated. This fungus produced alpha-amylase and alpha-glucosidase at low levels in the stationary culture filtrate. However, glucoamylase activity was not present and beta-glucosidase demonstrated strong activity in the stationary culture filtrate. These enzymes have previously been purified and characterized and the presence of beta-glucosidase suggests this fungus is saprotrophic abilities. A pattern for the T. matsutake carbohydrase production system is proposed, and the weak points of the fungus under artificial cultivation are discussed. In addition, the possibilities of using the potent beta-glucosidase activity as a glucose supply for artificial cultivation is discussed.