Analysis of maternal lineage in the "Fuji-York" strain of the large white pig using polymorphisms of the mitochondrial DNA major non-coding region

• Published in: Japanese Journal of Swine Science (Japan) Vol. 42; no. 3; pp. 130 - 138
• Main Authors: Ide, H.(Shizuoka-ken. Animal Husbandry Experiment Station, Fujinomiya (Japan)), Horiuchi, A, Chikyu, M, Terada, K, Okumura, N
• Format: Journal Article
• Language: Japanese
• Published: The Japanese Society of Swine Science 01.09.2005
• Subjects: ADN; ANALISIS HISTOCITOLOGICO; ANALYSE HISTOCYTOLOGIQUE; CERDO; CROSSBREDS; DESCENDANCE; DNA; HISTOCYTOLOGICAL ANALYSIS; Large White; MITOCHONDRIA; Mitochondrial DNA; MITOCHONDRIE; MITOCONDRIA; Non-coding region; POLIMORFISMO; POLYMORPHISM; POLYMORPHISME; PORCIN; PRODUCTOS DEL CRUZAMIENTO; PRODUIT DE CROISEMENT; PROGENIE; PROGENY; SWINE; Traceability
• ISSN: 0913-882X; 1881-655X
• DOI: 10.5938/youton.42.130

The traceability system using genetic markers is a useful method for discerning the breed, strain and individuality of farm products, and has been adapted for actual market foods such as pork, rice, strawberries and so forth. In this study, we examined the possibility of applying traceability to pork using mtDNA variation which discerns the maternal lineage of the porcine "Fuji-York" strain that was bred in our experiment station. First, we determined the sequences of the mitochondrial DNA (mtDNA) major non-coding region of 15 animals that represent the genetic background of the maternal lineage of the "Fuji-York" strain. In addition, mtDNA sequences were determined from 14 samples of the Large White breed introduced from three other prefectural animal breeding centers (Gifu, Tokushima, Toyama). We found that 10mtDNA haplotypes consisting of 26 nucleotide variations within the "Fuji-York" strain and the Large White breed, and two haplotypes (Ide5 and Ide8) within the "Fuji-York" strain had unique nucleotide substitution sites when compared to other haplotypes obtained from DNA databases. To apply PCR-RFLP analysis that distinguished these two haplotypes from others, we designed primers and selected restriction enzymes that recognized the unique nucleotide substitution of each haplotype. In fact, we could also distinguish the two unique haplotypes of the "Fuji-York" strain from the other eight types by employing PCR-RFLP analysis. To evaluate the rareness of these two haplotypes, we surveyed the mtDNA variations of an additional 198 pigs slaughtered at the meat center in Shizuoka Prefecture using PCR-RFLP analysis. It was revealed that the nucleotide substitutions of these two haplotypes were rare, because haplotype Ide5 and Ide8 were detected in only three and no samples respectively, out of a total of 198 individuals. According to the uniqueness of the two haplotypes, we are able to discern pork with the "Fuji-York" strain by constructing the maternal population that has rare types of mtDNA.