Deduced amino acid sequences at the haemagglutinin cleavage site of avian influenza A viruses of H5 and H7 subtypes

• Published in: Archives of virology Vol. 130; no. 1/2; pp. 209 - 217
• Main Authors: Wood, G.W, McCauley, J.W, Bashiruddin, J.B, Alexander, D.J
• Format: Journal Article
• Language: English
• Published: Austria 1993
• Subjects: Amino Acid Sequence; amino acid sequences; Animals; Base Sequence; Birds; Chick Embryo; Chickens; Hemagglutinin Glycoproteins, Influenza Virus; Hemagglutinins, Viral - genetics; Influenza A virus; Influenza A virus - classification; Influenza A virus - genetics; Molecular Sequence Data; nucleotide sequences; Oligodeoxyribonucleotides; Polymerase Chain Reaction; sequence analysis; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; strains; Turkeys; Viral Envelope Proteins - genetics; viral hemagglutinins; virulence
• ISSN: 0304-8608; 1432-8798

The amino acid sequences at the hemagglutinin cleavage sites of 9 avian influenza A viruses of H 5 subtype (5 high and 4 low pathogenicity for chickens) and 21 of H 7 subtype (13 high and 8 low pathogenicity for chickens) were determined by direct RNA sequencing, PCR amplification sequencing or both. None of the viruses of low pathogenicity had multiple basic amino acids at the cleavage site. All highly pathogenic viruses had an insert of basic amino acids at the cleavage site, except A/chicken/Scotland/59 (H5N1) for which the multiple basic amino acids appeared as substitutions and not insertions. All highly pathogenic viruses examined conformed to the amino acid motif of R-X-R/K-R at the cleavage site which is considered to be essential for high pathogenicity in chickens, with the notable exception of highly pathogenic virus A/turkey/England/50-92/91 (H5N1) which had the sequence R-K-R-K-T-R adjacent to the cleavage site.