Partial sequence analysis of Hibiscus syriacus cDNA clones

To study the genome of Hibiscus syriacus, we generated 110 expressed sequence tags (ESTs) from cDNA leaf library. A homology search of these 110 ESTs against the NCBI (National Center for Biotechnology) non-redundant protein database revealed that 19 clones (17.3%) were significantly similar to the...

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Bibliographic Details
Published inJournal of the Korean Society for Horticultural Science Vol. 39; no. 3
Main Authors Um, B.Y, Park, C.H. (Korea University, Seoul (Korea Republic). Department of Horticultural Science), Ok, S.H, Chung, Y.S, Shin, J.S. (Korea University, Seoul (Korea Republic). Graduate School of Biotech.)
Format Journal Article
LanguageKorean
Published 1998
Subjects
ADN
ANALISIS DE TEJIDOS
ANALISIS MICROBIOLOGICO
ANALYSE DE TISSUS
ANALYSE MICROBIOLOGIQUE
ARN
CLONE
CLONES
DNA
GENE
GENES
GENOMAS
GENOME
GENOMES
HIBISCUS SYRIACUS
MICROBIOLOGICAL ANALYSIS
PROTEINAS
PROTEINE
PROTEINS
RNA
TISSUE ANALYSIS
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Summary:To study the genome of Hibiscus syriacus, we generated 110 expressed sequence tags (ESTs) from cDNA leaf library. A homology search of these 110 ESTs against the NCBI (National Center for Biotechnology) non-redundant protein database revealed that 19 clones (17.3%) were significantly similar to the protein coding sequences. Nine clones had relatively high homologies to retrovirus-related pol protein genes. Ten c DNAs had high degree of sequence similarities when compared with genes from other organisms. The remaining cDNA clones seem to be new genes not only in Hibiscus syriacus genome but also in all organisms. The arerage size of insert was about 2.66 kb (SD = 1.66 kb). In this paper, we also report an efficient and rapid method for RNA isolation from high polysaccharide-containing material, like Hibiscus syriacus.
Bibliography:1999000669
F30
2001000050
ISSN:0253-6498