ISSR Study on Genetic Relationship of Genus Paulownia

利用ISSR分子标记对泡桐属21份种、变种及变型材料进行亲缘关系分析。结果表明：从100个ISSR引物中筛选出9个扩增产物电泳后带型清晰可辨的引物,共扩增出85条DNA片段,其中多态性带74条,多态位点百分率为87.05%,反映出泡桐属不同种质间遗传差异明显,其丰富的多态性与供试材料广泛的种质代表性有关;UPGMA法将研究材料分成毛泡桐组、白花泡桐组和川泡桐组3大组,地理分布较近的种质聚类在一起,表现出较密切的亲缘关系;在对兰考泡桐、山明泡桐、白花兰考泡桐、楸叶泡桐、圆冠泡桐、亮叶毛泡桐、毛泡桐、白花泡桐、川泡桐的亲缘关系分析及分类处理上,基本上与形态学、细胞学、同工酶的研究结果一致。最后对泡...

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Bibliographic Details
Published inLinye kexue (1979) Vol. 49; no. 1
Main Authors Mo Wenjuan, China Paulownia Research Center, Zhengzhou(China), Fu Jianmin, China Paulownia Research Center, Zhengzhou(China), Qiao Jie, China Paulownia Research Center, Zhengzhou(China)
Format Journal Article
LanguageChinese
Published 01.01.2013
Subjects
AMELIORATION DES PLANTES
FITOMEJORAMIENTO
GENERA
GENETIC VARIATION
GENETICA
GENETICS
GENETIQUE
GENRE
http://www.fao.org/aos/agrovoc#c_15975
http://www.fao.org/aos/agrovoc#c_3213
http://www.fao.org/aos/agrovoc#c_3222
http://www.fao.org/aos/agrovoc#c_5956
PLANT BREEDING
VARIACION GENETICA
VARIATION GENETIQUE
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Summary:利用ISSR分子标记对泡桐属21份种、变种及变型材料进行亲缘关系分析。结果表明：从100个ISSR引物中筛选出9个扩增产物电泳后带型清晰可辨的引物,共扩增出85条DNA片段,其中多态性带74条,多态位点百分率为87.05%,反映出泡桐属不同种质间遗传差异明显,其丰富的多态性与供试材料广泛的种质代表性有关;UPGMA法将研究材料分成毛泡桐组、白花泡桐组和川泡桐组3大组,地理分布较近的种质聚类在一起,表现出较密切的亲缘关系;在对兰考泡桐、山明泡桐、白花兰考泡桐、楸叶泡桐、圆冠泡桐、亮叶毛泡桐、毛泡桐、白花泡桐、川泡桐的亲缘关系分析及分类处理上,基本上与形态学、细胞学、同工酶的研究结果一致。最后对泡桐属的部分植物的分类问题进行讨论。同时发现部分泡桐的一些特异性条带,可以进一步转化为SCAR标记。 In this study, 21 species, varieties and forma in Paulownia were used to detect the genetic relationships by using inter-simple sequence repeats （ISSR） markers. The results showed that： 1 ） A total of 85 DNA fragments were amplified using 9 out of 100 ISSR primers with unambiguous unique polymorphic bands, among which 74 DNA bands were polymorphic （ PPB = 87.05 % ） , indicating significant genetic differentiation among Paulownia accessions tested ; 2） The dendrogram was constructed with MEGA3.1 statistical package using UPGMA （the unweighted pair-group method with arithmetic average）, with which Paulownia was roughly divided into three main groups of Paulownia tomen
Bibliography:2014001303
F30
ISSN:1001-7488
DOI:10.11707/j.1001-7488.20130110