Construction of the plasmid reference molecule for detection of transgenic soybean MON89788

[Objective] The aim was to construct a plasmid reference molecule (PRM) for detection of transgenic soybean MON89788. [Method] the lectin gene sequence, 3’-junction and 5’-junction sequence between host plant DNA integrated DNA of MON89788 soybean were amplified independently, and the three fragment...

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Published inHunan agricultural science & technology newsletter : HASTN Vol. 11; no. 5
Main Authors Li Feiwu, Jilin Academy of Agriculture Sciences, Changchun (China), Shao Gaige, Jilin Academy of Agriculture Sciences, Changchun (China), Xing Zhenjuan, Jilin Academy of Agriculture Sciences, Changchun (China)
Format Journal Article
LanguageChinese
Published 01.10.2010
Subjects
GENETICALLY MODIFIED ORGANISMS
http://www.fao.org/aos/agrovoc#c_14477
http://www.fao.org/aos/agrovoc#c_16014
http://www.fao.org/aos/agrovoc#c_34285
ORGANISME GENETIQUEMENT MODIFIE
ORGANISMOS MODIFICADOS GENETICAMENTE
PLASMIDE
PLASMIDIOS
PLASMIDS
SOJA
SOYBEANS
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Summary:[Objective] The aim was to construct a plasmid reference molecule (PRM) for detection of transgenic soybean MON89788. [Method] the lectin gene sequence, 3’-junction and 5’-junction sequence between host plant DNA integrated DNA of MON89788 soybean were amplified independently, and the three fragments were cloned into the cloning vector pMD18-T in order through molecular manipulation method to construct pMD-LM3M5, the applicability of the constructed novel PRM was tested. [Result] Sequencing confirmation result showed that the PRM was 3 700 bp in length, containing 1 029bp of recombined DNA fragment. The limits of qualitative detection of the PRM were 10 copies. [Conclusion] The PRM constructed in this study was suitable for the identification of MON89788 event.
Bibliography:F60
2011000662
ISSN:1009-4229