Kinetic analysis of Saccharomyces cerevisiae cell immobilization onto ceramic supports

• Published in: Polish journal of food and nutrition sciences Vol. 11; no. 3; pp. 51 - 56
• Main Authors: Cibis, E. (Wroclaw University of Economics (Poland). Dept. of Bioprocess Engineering), Zboromirska-Wnukiewicz, B. (Electrotechnical Institute, Wroclaw (Poland). Div. of Electrotechnology and Materials Science), Garncarek, Z, Gasperowicz, A, Krzywonos, M
• Format: Journal Article
• Language: English
• Published: Olsztyn Polish Academy of Sciences 2002; Institute of Animal Reproduction
• Subjects: ALCOHOLIC FERMENTATION; Biological and medical sciences; Biotechnology; CELLS; CELLULE; CELULAS; CERAMICA; CERAMICS; CERAMIQUE; ETANOL; ETHANOL; FERMENTACION ALCOHOLICA; FERMENTATION ALCOOLIQUE; Fundamental and applied biological sciences. Psychology; IMMOBILISATION; IMMOBILIZATION; Immobilization of organelles and whole cells; Immobilization techniques; INMOVILIZACION; LEVADURA; LEVURE; Methods. Procedures. Technologies; SACCHAROMYCES CEREVISIAE; YEASTS; Fungi; Yeast; Adsorption; Alcoholic fermentation; Silylation; Ascomycetes; Aluminium; Kinetics; Ceramic materials; Entrapped microorganism; Saccharomyces cerevisiae; Thallophyta
• ISSN: 1230-0322; 2083-6007

Saccharomyces cerevisiae yeast was immobilized on Al-70 ceramic supports with and without changing their surface zeta-potentials via silylation. Preliminary ethanol fermentation was carried out with yeast cells immobilized on a silylated support. The immobilization technique involved recirculation of the yeast suspension through a column packed with ceramic support material. With a low initial biomass concentration in the suspension, cell immobilization was a single-stage process where the amout of yeast cells fixed on the ceramic bed can be expressed as an expotential time function. With high initial biomass content immobilization was two-stage process. The increase of the initial yeast concentration brought about decrease of the specific immobilization rate in the single stage process and in the first phase of the two-stage process. Sililation accounted for a noticeable increase of specific immobilization rate both in single-stage process and in the first phase of the two-stage process. Ethanol fermentation involving yeast cells immobilized on a silylated ceramic carrier had an efficiency which accounted for 83.5 percent of the theoretical efficiency