Evidence for extensive parallelism but divergent genomic architecture of adaptation along altitudinal and latitudinal gradients in Populus trichocarpa

• Published in: The New phytologist Vol. 209; no. 3; pp. 1240 - 1251
• Main Authors: Holliday, Jason A, Zhou, Lecong, Bawa, Rajesh, Zhang, Man, Oubida, Regis W
• Format: Journal Article
• Language: English
• Published: England Academic Press 01.02.2016; New Phytologist Trust; Wiley Subscription Services, Inc
• Subjects: Adaptation, Physiological - genetics; alleles; Altitude; climate; Climate adaptation; Cluster Analysis; CpG Islands - genetics; gene flow; Gene Ontology; Genetic Loci; Genome, Plant; genomic architecture; Genomics; Islands; Latitude; local adaptation; loci; parallel adaptation; phenotype; Polymorphism, Single Nucleotide - genetics; Populus; Populus - genetics; Populus - physiology; Populus balsamifera subsp. trichocarpa; Populus trichocarpa; sequence capture; trees; local adaptation; sequence capture; genomic architecture; Populus trichocarpa; parallel adaptation
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.13643

Adaptation to climate across latitude and altitude reflects shared climatic constraints, which may lead to parallel adaptation. However, theory predicts that higher gene flow should favor more concentrated genomic architectures, which would lead to fewer locally maladapted recombinants. We used exome capture to resequence the gene space along a latitudinal and two altitudinal transects in the model tree Populus trichocapra. Adaptive trait phenotyping was coupled with FST outlier tests and sliding window analysis to assess the degree of parallel adaptation as well as the genomic distribution of outlier loci. Up to 51% of outlier loci overlapped between transect pairs and up to 15% of these loci overlapped among all three transects. Genomic clustering of adaptive loci was more pronounced for altitudinal than latitudinal transects. In both altitudinal transects, there was a larger number of these ‘islands of divergence’, which were on average longer and included several of exceptional physical length. Our results suggest that recapitulation of genetic clines over latitude and altitude involves extensive parallelism, but that steep altitudinal clines generate islands of divergence. This suggests that physical proximity of genes in coadapted complexes may buffer against the movement of maladapted alleles from geographically proximal but climatically distinct populations.