Combined effects of different CO₂ levels and N sources on the diazotrophic cyanobacterium Trichodesmium

• Published in: Physiologia plantarum Vol. 152; no. 2; pp. 316 - 330
• Main Authors: Eichner, Meri, Kranz, Sven A, Rost, Björn
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.10.2014; Wiley Subscription Services, Inc
• Subjects: Acclimatization - drug effects; Acclimatization - radiation effects; adenosine triphosphate; biomass production; carbon; carbon dioxide; Carbon Dioxide - pharmacology; chlorophyll; Chlorophyll - metabolism; Circadian Rhythm - drug effects; Circadian Rhythm - radiation effects; climate change; Cyanobacteria - drug effects; Cyanobacteria - growth & development; Cyanobacteria - physiology; Cyanobacteria - radiation effects; diurnal variation; Ecophysiology, Stress and Adaptation; energy; environmental factors; Fluorescence; Light; nitrogen; Nitrogen - pharmacology; nitrogen content; nitrogen fixation; Nitrogen Fixation - drug effects; Nitrogen Fixation - radiation effects; nitrogenase; oxygen; Oxygen - metabolism; Particulate Matter - analysis; photosynthesis; phytoplankton; Trichodesmium erythraeum
• ISSN: 0031-9317; 1399-3054
• DOI: 10.1111/ppl.12172

To predict effects of climate change and possible feedbacks, it is crucial to understand the mechanisms behind CO₂ responses of biogeochemically relevant phytoplankton species. Previous experiments on the abundant N₂ fixers Trichodesmium demonstrated strong CO₂ responses, which were attributed to an energy reallocation between its carbon (C) and nitrogen (N) acquisition. Pursuing this hypothesis, we manipulated the cellular energy budget by growing Trichodesmium erythraeum IMS101 under different CO₂ partial pressure (pCO₂) levels (180, 380, 980 and 1400 µatm) and N sources (N₂ and NO₃ ⁻). Subsequently, biomass production and the main energy‐generating processes (photosynthesis and respiration) and energy‐consuming processes (N₂ fixation and C acquisition) were measured. While oxygen fluxes and chlorophyll fluorescence indicated that energy generation and its diurnal cycle was neither affected by pCO₂ nor N source, cells differed in production rates and composition. Elevated pCO₂ increased N₂ fixation and organic C and N contents. The degree of stimulation was higher for nitrogenase activity than for cell contents, indicating a pCO₂ effect on the transfer efficiency from N₂ to biomass. pCO₂‐dependent changes in the diurnal cycle of N₂ fixation correlated well with C affinities, confirming the interactions between N and C acquisition. Regarding effects of the N source, production rates were enhanced in NO₃ ⁻ grown cells, which we attribute to the higher N retention and lower ATP demand compared with N₂ fixation. pCO₂ effects on C affinity were less pronounced in NO₃ ⁻ users than N₂ fixers. Our study illustrates the necessity to understand energy budgets and fluxes under different environmental conditions for explaining indirect effects of rising pCO₂.