Identification of novel genetic markers and evaluation of genetic structure in a population of Japanese crested ibis
Japanese population of the Japanese crested ibis Nipponia nippon was founded by five individuals gifted from the People's Republic of China. In order to exactly evaluate genetic structure, we first performed development of novel genetic makers using 89 microsatellite primer pairs of related spe...
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Published in | Animal science journal Vol. 85; no. 4; pp. 356 - 364 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Australia
Blackwell
01.04.2014
Blackwell Publishing Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | Japanese population of the Japanese crested ibis Nipponia nippon was founded by five individuals gifted from the People's Republic of China. In order to exactly evaluate genetic structure, we first performed development of novel genetic makers using 89 microsatellite primer pairs of related species for cross‐amplification. Of these, only three primer pairs were useful for the genetic markers. Additionally, we sequenced allelic PCR products of these three markers together with 10 markers previously identified. Most markers showed typical microsatellite repeat units, but two markers were not simple microsatellites. Moreover, over half of the markers did not have the same repeat units as those of the original species. These results suggested that development of novel genetic markers in this population by cross‐amplification is not efficient, partly because of low genetic diversity. Furthermore, the cluster analysis by STRUCTURE program using 17 markers showed that the five founders were divided into two clusters. However, the genetic relationships among the founders indicated by the clustering seemed to be questionable, because the analysis relied largely on a small number of triallelic markers, in spite of the addition of the three useful markers. Therefore, more efficient methods for identifying large numbers of single nucleotide polymorphisms are desirable. |
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Bibliography: | http://dx.doi.org/10.1111/asj.12155 Japanese Crested Ibis Project, Center for Transdisciplinary Research, Niigata University, Japan istex:1A476C485428E275D5ABC04B8D649EE925CD5996 ark:/67375/WNG-BS9CQK0R-Z ArticleID:ASJ12155 Japanese Ministry of the Environment, Sado city ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 1344-3941 1740-0929 |
DOI: | 10.1111/asj.12155 |