Bovine herpesvirus-1 (BHV-1) recombinant expressing pseudorabies virus (PrV) glycoproteins B and C induces type 1 immune response in BALB/c mice

• Published in: Journal of Veterinary Medical Science Vol. 64; no. 7; pp. 589 - 596
• Main Authors: Takashima, Y. (Tokyo Univ. (Japan)), Nagane, N, Hushur, O, Matsumoto, Y, Otsuka, H
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.07.2002; Japan Science and Technology Agency
• Subjects: Animals; Antibodies, Viral - immunology; AUJESZKY VIRUS; BHV-1; BOVINE HERPESVIRUS; Bovine herpesvirus 1; DNA, Recombinant - genetics; Genetic Engineering; Herpesvirus 1, Bovine - genetics; Herpesvirus 1, Bovine - physiology; Herpesvirus 1, Suid - genetics; Herpesvirus 1, Suid - immunology; Herpesvirus 1, Suid - physiology; Hypersensitivity, Delayed - immunology; IgG1/IgG2a; IMMUNE RESPONSE; Immunization; Immunoglobulin G - immunology; MICE; Mice, Inbred BALB C; Organ Specificity; PrV; Pseudorabies virus; Th1 Cells - immunology; Time Factors; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.64.589

Bovine herpesvirus 1 (BHV-1) attached poorly and penetrated into a mouse cell line, BALB 3T3/A31, but a recombinant BHV-1/TF7-6, which expresses pseudorabies virus (PrV) gB and gC genes, did attach and penetrated into cells more efficiently. In this study the gene green fluorescent protein (GFP) has been integrated into genome of BHV-1/TF7-6 and its parental line of BHV-1. When the mouse mesenteries were incubated in vitro and infected with BHV-1/TF7-6/GFP, strong fluorescence was observed while BHV-1/GFP infection hardly demonstrated fluorescence, suggesting that BHV-1 recombinant expressing PrV gB and gC can infect mouse tissue cells more efficiently than the parental BHV-1 does. When BALB/c mice were inoculated with purified BHV-1/TF7-6 or its parental BHV-1, the former induced lower level of anti-BHV-1 immunogloblin G (IgG) than the latter did. When sub-classes of anti-BHV-1 IgG were analyzed, it was found that mice immunized with BHV-1/TF7-6 or the parental BHV-1 demonstrated the same level of IgG2a. Since anti-BHV-1 IgG1level was lower in mice inoculated with BHV-1/TF7-6, the IgG2a:IgG1 ratio was higher in BHV-1/TF7-6 inoculated mice than in the parental BHV-1 inoculated ones. These results indicate that BHV-1/TF7-6 induces type I predominant immune to BALB/c mice.