modified chromosome squash technique to determine mitotic activity in primed mericarps

Mericarps of 'Moss Curled' parsley (Petroselinum crispum Mill. Nym. ex A. W. Hill) were hydroprimed (1 or 3 days in water at 20°C) or osmotically primed in polyethylene glycol (-1.0 MPa for 7 or 21 days at 20°C). Extracted whole embryos then were subjected to a modified chromosome squash t...

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Bibliographic Details
Published inSeed technology Vol. 27; no. 1; pp. 101 - 103
Main Authors Olszewski, M.W, Pill, W.G, Pizzolato, T.D
Format Journal Article
LanguageEnglish
Published 2005
Subjects
anaphase
chromosome squash technique
chromosomes
embryo (plant)
mitosis
parsley
Petroselinum crispum
polyethylene glycol
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Summary:Mericarps of 'Moss Curled' parsley (Petroselinum crispum Mill. Nym. ex A. W. Hill) were hydroprimed (1 or 3 days in water at 20°C) or osmotically primed in polyethylene glycol (-1.0 MPa for 7 or 21 days at 20°C). Extracted whole embryos then were subjected to a modified chromosome squash technique which sequentially involved fixing, staining, tissue squashing, and chromosomal viewing. The technique quantified relative embryonic mitotic activity. The number of cells per embryo with late anaphase activity was greatest for 3 day hydroprimed mericarps (15), then 7 or 21 day osmotic primed mericarps (3), with 1 day hydroprimed mericarps showing no mitotic activity.
ISSN:1096-0724