Development of chitosan conjugated DNA vaccine against nodavirus in Macrobrachium rosenbergii (De Man, 1879)

• Published in: Journal of fish diseases Vol. 37; no. 9; pp. 815 - 824
• Main Authors: Ramya, V L, Sharma, R, Gireesh‐Babu, P, Patchala, S R, Rather, A, Nandanpawar, P C, Eswaran, S
• Format: Journal Article
• Language: English
• Published: England Blackwell Science 01.09.2014; Blackwell Publishing Ltd
• Subjects: agarose; Animals; Aquaculture; atomic force microscopy; chitosan; Chitosan - administration & dosage; Chitosan - adverse effects; DNA; Freshwater; gel electrophoresis; genes; Macrobrachium rosenbergii; Microscopy, Atomic Force; Nanoconjugates - administration & dosage; Nanoconjugates - adverse effects; nanoparticles; Nodaviridae; Nodaviridae - genetics; Nodaviridae - immunology; Nodavirus; oral administration; Palaemonidae - immunology; Palaemonidae - virology; plasmids; recombinant vaccines; shrimp; Vaccines, DNA - administration & dosage; Vaccines, DNA - adverse effects; Viral Vaccines - administration & dosage; Viral Vaccines - adverse effects; viruses; zeta potential; nodavirus; chitosan; Macrobrachium rosenbergii; DNA
• ISSN: 0140-7775; 1365-2761
• DOI: 10.1111/jfd.12179

The protective efficacy of a DNA construct containing extra small virus antisense (XSVAS) gene of nodavirus encapsulated with chitosan nanoparticles (NPs) was investigated in giant freshwater prawn Macrobrachium rosenbergii (De Man, 1879). The delivery was carried out using oral and immersion methods. A plasmid concentration of 100 ng μL⁻¹ when conjugated with chitosan NPs was found to be more effective in increasing the survivability of the infected prawn. The particle mean size, zeta potential and loading efficiency percentage were 297 nm, 27 mV and 85%, respectively. The ability of the chitosan to form a complex with the plasmid was studied by agarose gel electrophoresis. The NPs were characterized by atomic force microscopy (AFM). Persistence study showed the presence of the DNA construct up to 30th day post‐treatment. The oral treatment was found to be better than the immersion treatment for delivery of the chitosan‐conjugated DNA construct. This is probably the first report on the delivery of nanoconjugated DNA construct in M. rosenbergii, against nodavirus.