Cryptosporidium hominis Subtypes and Enterocytozoon bieneusi Genotypes in HIV‐Infected Persons in Ibadan, Nigeria

• Published in: Zoonoses and public health Vol. 61; no. 4; pp. 297 - 303
• Main Authors: Ayinmode, A. B, Zhang, H, Dada‐Adegbola, H. O, Xiao, L
• Format: Journal Article
• Language: English
• Published: Germany Blackwell 01.06.2014; Blackwell Publishing Ltd
• Subjects: Adult; Child, Preschool; cryptosporidiosis; Cryptosporidiosis - epidemiology; Cryptosporidiosis - parasitology; Cryptosporidium; Cryptosporidium - genetics; Cryptosporidium hominis; developing countries; diarrhoea; Enterocytozoon; Enterocytozoon - genetics; Enterocytozoon bieneusi; epidemiology; feces; Female; genes; Genotype; Genotype & phenotype; HIV; HIV Infections - complications; HIV Infections - epidemiology; HIV Infections - microbiology; HIV Infections - parasitology; Human immunodeficiency virus; Humans; internal transcribed spacers; Logistic Models; Male; microsporidiosis; Microsporidiosis - epidemiology; Microsporidiosis - microbiology; Middle Aged; Molecular Sequence Data; new species; opportunistic infection; pathogens; patients; people; polymerase chain reaction; Risk factors; sequence analysis; therapeutics; Zoonoses; Nigeria; United Kingdom; Peru; Nigeria, Ibadan; Cryptosporidium; epidemiology; HIV; microsporidiosis; Enterocytozoon bieneusi; cryptosporidiosis; diarrhoea; opportunistic infection
• ISSN: 1863-1959; 1863-2378
• DOI: 10.1111/zph.12072

Cryptosporidium and Enterocytozoon are common opportunistic pathogens in HIV+ patients in developing countries, especially those do not have access to antiretroviral therapy. To determine the distribution of genotypes/subtypes of Cryptosporidium and Enterocytozoon bieneusi, faecal specimens were collected from 132 HIV+ persons attending a tertiary hospital in Ibadan, Nigeria. By polymerase chain reaction, eight and ten patients were identified as positive for Cryptosporidium spp. and E. bieneusi, respectively. Seven of the Cryptosporidium specimens were identified as C. hominis, while the remaining one as the new species C. viatorum recently identified in the United Kingdom. DNA sequencing of the 60‐kDa glycoprotein gene showed that the C. hominis belonged to three common subtype families: Ia (in three patients), Ib (in one patient) and Ie (in one patient). In contrast, DNA sequencing of the E. bieneusi internal transcribed spacer products showed the occurrence of genotypes associated with both humans (Peru 8 in one patient, Nig2 in two patients and a new genotype in one patient) and animals (D in one patient and Type IV in five patients). Low CD4+ cell count was identified as a risk factor for both cryptosporidiosis and microsporidiosis.