NMR Metabolomics to Revisit the Tobacco Mosaic Virus Infection in Nicotiana tabacum Leaves

• Published in: Journal of natural products (Washington, D.C.) Vol. 69; no. 5; pp. 742 - 748
• Main Authors: Choi, Y.H, Kim, H.K, Linthorst, H.J.M, Hollander, J.G, Lefeber, A.W.M, Erkelens, C, Nuzillard, J.M, Verpoorte, R
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.05.2006; Glendale, AZ American Society of Pharmacognosy
• Subjects: alpha-Linolenic Acid - metabolism; Biological and medical sciences; Caffeic Acids - metabolism; Chlorogenic Acid - analogs & derivatives; Fundamental and applied biological sciences. Psychology; Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control; metabolomics; Models, Biological; Molecular Structure; Nicotiana - metabolism; Nicotiana - virology; Nicotiana tabacum; nuclear magnetic resonance spectroscopy; Nuclear Magnetic Resonance, Biomolecular; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - virology; Plant Leaves - chemistry; Plant Leaves - metabolism; plant viruses; Plant viruses and viroids; Quinic Acid - analogs & derivatives; Quinic Acid - metabolism; Terpenes - metabolism; Tobacco mosaic virus; Tobacco Mosaic Virus - pathogenicity; Metabolite; Tobamovirus; NMR spectrometry; Nicotiana tabacum; Induced resistance; Host virus relation; Tobacco mosaic virus; Dicotyledones; Metabonomics; Sesquiterpenes; Angiospermae; Qualitative analysis; Solanaceae; Terpenoid; Polyunsaturated fatty acid; Plant leaf; Metabolism; Two dimension spectrometry; Virus; Infection; Linolenic acid; Plant origin; Diterpene; Spermatophyta; Chemometrics; Principal component analysis
• ISSN: 0163-3864; 1520-6025
• DOI: 10.1021/np050535b

Tobacco mosaic virus (TMV) infection of tobacco is a well-known and extensively studied model system for which a number of genes and proteins involved in the systemic acquired resistance (SAR) have been characterized. Little is known about the metabolic changes connected with the infection and SAR. Here we describe the use of NMR spectroscopy in combination with multivariate data analysis to study the metabolic changes. Particularly 2-D NMR methods, such as 2-D J-resolved spectra and their projected spectra, are shown to be powerful tools in the metabolomic studies. The macroscopic view of the metabolomes obtained by NMR spectroscopy of crude extracts enabled the identification of a series of totally different metabolites that seem connected with resistance, such as the clearly increased 5-caffeoylquinic acid, alpha-linolenic acid analogues, and sesqui- and diterpenoids in the infected plant parts.