transgenic Arabidopsis plant system, pER8-GFP, as a powerful tool in searching for natural product estrogen-agonists/antagonists

• Published in: Journal of natural products (Washington, D.C.) Vol. 68; no. 7; pp. 971 - 973
• Main Authors: Chang, F.R, Hayashi, K, Chua, N.H, Kamio, S, Huang, Z.Y, Nozaki, H, Wu, Y.C
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.07.2005; Glendale, AZ American Society of Pharmacognosy
• Subjects: agonists; antagonists; Arabidopsis; Arabidopsis thaliana; Biological and medical sciences; Biological Factors - isolation & purification; Biological Factors - pharmacology; Estrogen Antagonists - isolation & purification; Estrogens - agonists; Estrogens - isolation & purification; General pharmacology; Glycine max; green fluorescent protein; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phytoestrogens - isolation & purification; Phytoestrogens - pharmacology; plant biochemistry; plant estrogens; plant genetics; Plants, Genetically Modified; Pueraria lobata; reporter genes; Structure-Activity Relationship; transgenic plants; Asia; China; Estrogen receptor; Agonist; Genistein; Antiestrogen; Estrogen; Antihormone; Estradiol; Flavonoid; Isoflavone derivatives; Medicinal plant; Phytoestrogen; Reporter gene; Folk medicine; Structure activity relation; Cruciferae; Dicotyledones; Angiospermae; Transgenic plant; Antagonist; Human; Mimetic hormone; Arabidopsis; Pharmacognosy; Natural compound; Glycine max; In vitro; Leguminosae; Plant origin; Green fluorescent protein; Spermatophyta; Daidzein; Hormonal receptor

The transgenic Arabidopsis plant system, pER8-GFP, may be used as a powerful tool in searching for natural estrogen-agonists/antagonists. Among selected plant extracts and natural products, the method was able to distinguish active extracts (e.g., Glycine max and Pueraria lobata) and pure compounds...