transgenic Arabidopsis plant system, pER8-GFP, as a powerful tool in searching for natural product estrogen-agonists/antagonists

• Published in: Journal of natural products (Washington, D.C.) Vol. 68; no. 7; pp. 971 - 973
• Main Authors: Chang, F.R, Hayashi, K, Chua, N.H, Kamio, S, Huang, Z.Y, Nozaki, H, Wu, Y.C
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.07.2005; Glendale, AZ American Society of Pharmacognosy
• Subjects: agonists; antagonists; Arabidopsis; Arabidopsis thaliana; Biological and medical sciences; Biological Factors - isolation & purification; Biological Factors - pharmacology; Estrogen Antagonists - isolation & purification; Estrogens - agonists; Estrogens - isolation & purification; General pharmacology; Glycine max; green fluorescent protein; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phytoestrogens - isolation & purification; Phytoestrogens - pharmacology; plant biochemistry; plant estrogens; plant genetics; Plants, Genetically Modified; Pueraria lobata; reporter genes; Structure-Activity Relationship; transgenic plants; Asia; China; Estrogen receptor; Agonist; Genistein; Antiestrogen; Estrogen; Antihormone; Estradiol; Flavonoid; Isoflavone derivatives; Medicinal plant; Phytoestrogen; Reporter gene; Folk medicine; Structure activity relation; Cruciferae; Dicotyledones; Angiospermae; Transgenic plant; Antagonist; Human; Mimetic hormone; Arabidopsis; Pharmacognosy; Natural compound; Glycine max; In vitro; Leguminosae; Plant origin; Green fluorescent protein; Spermatophyta; Daidzein; Hormonal receptor
• ISSN: 0163-3864; 1520-6025
• DOI: 10.1021/np050121i

The transgenic Arabidopsis plant system, pER8-GFP, may be used as a powerful tool in searching for natural estrogen-agonists/antagonists. Among selected plant extracts and natural products, the method was able to distinguish active extracts (e.g., Glycine max and Pueraria lobata) and pure compounds (e.g., 17beta-estradiol (1), genistein (10), and daidzein (11)) and also to distinguish effects of structural changes on activity. Thus, this rapid sensitive system was proven to be suitable for the discovery of natural products with estrogenic activity.