Estimating growth kinetics of Penicillium chrysogenum through the use of respirometry

• Published in: Journal of chemical technology and biotechnology (1986) Vol. 72; no. 3; pp. 207 - 212
• Main Authors: Goudar, C.T, Strevett, K.A
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.07.1998; Wiley
• Subjects: Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Gas absorption; Growth kinetics; Mathematical models; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; microbial kinetics; Oxygen; oxygen consumption; oxygen uptake; Parameter estimation; Penicillium chrysogenum; respirometry; Saturation (materials composition); Fungi; Microorganism growth; Respirometry; Microorganism culture; Oxygen consumption; Fungi Imperfecti; Kinetics; Mathematical model; Thallophyta; Penicillium chrysogenum
• ISSN: 0268-2575; 1097-4660
• DOI: 10.1002/(SICI)1097-4660(199807)72:3<207::AID-JCTB899>3.0.CO;2-B

The applicability of respirometry to characterize fungal growth kinetics was studied using Penicillium chrysogenum as the model microorganism. Oxygen uptake data were used in conjunction with a nonlinear parameter estimation technique to determine the kinetic parameters associated with microbial growth. The theoretical model that used the Contois equation for microbial growth was able to accurately describe experimental oxygen uptake data. Penicillium chrysogenum was characterized by average values of 0·195 h−1 and 4·13 for the maximum specific growth rate, μmax, and the Contois saturation coefficient, Kc, respectively. The average true growth yield was 0·405 mg mg−1. As only oxygen uptake information is required for biokinetic parameter estimation, respirometry is a very convenient tool for studying microbial growth as high quality experimental data can be obtained with relatively little experimental effort. © 1998 SCI