Western Flower Thrips (Frankliniella occidentalis) Transmits Maize Chlorotic Mottle Virus

• Published in: Journal of phytopathology Vol. 162; no. 7-8; pp. 532 - 536
• Main Authors: Zhao, Mingfu, Ho, Honhing, Wu, Yixin, He, Yueqiu, Li, Mengjiao
• Format: Journal Article
• Language: English
• Published: Berlin Blackwell Science 01.08.2014; Blackwell Publishing Ltd; Wiley Subscription Services, Inc
• Subjects: China; corn; Frankliniella occidentalis; greenhouses; Maize chlorotic mottle virus; maize diseases; necrosis; nucleotides; pathogens; reverse transcriptase polymerase chain reaction; seedlings; seeds; sequence analysis; viruses; western flower thrips vectors; Zea mays; Thailand
• ISSN: 0931-1785; 1439-0434
• DOI: 10.1111/jph.12217

Maize chlorotic mottle virus (MCMV) is one of the co‐infection pathogens that cause corn (maize) lethal necrosis, but the transmission mechanism of MCMV is not yet clear. In order to determine the ability of western flower thrips (Frankliniella occidentalis; WFT) to transmit MCMV, imported maize seeds from Thailand were germinated in an insect‐free greenhouse and the seedlings were tested for the transmission by WFT of chlorotic mottle virus disease. The thrips (WFT), starved for 48 h then allowed to feed for 30 min on maize plants infected with MCMV or asymptomatic maize plants, were transferred to healthy seedlings. After 35 days, the seedlings with WFT from diseased maize plants showed chlorotic mottle symptoms, whereas seedlings with WFT from asymptomatic maize plants remained healthy. A single band of 711 bp was amplified by RT‐PCR using primers MCMV‐F/MCMV‐R from the MCMV‐infected plants and WFT collected from the diseased plants. Sequencing of the amplified product and further sequence comparison indicated that the two viruses from both sources showed 99% similarity of nucleotides and they should be regarded as identical. In addition, isometric particles c. 30 nm in diameter, characteristic of MCMV, were found in the WFT samples from diseased maize plants. Thus, it is concluded that WFT transmits MCMV. Our findings suggest that corn lethal necrosis disease can be controlled or minimized by the eradication of WFT from the field or greenhouses.