Stimulation of the blue light phototropic receptor NPH1 causes a transient increase in cytosolic Ca2

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 96; no. 23; pp. 13554 - 13559
• Main Authors: Baum, G, Long, J.C, Jenkins, G.I, Trewavas, A.J
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 09.11.1999; National Acad Sciences; National Academy of Sciences; The National Academy of Sciences
• Subjects: Aequorin; Arabidopsis - cytology; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis - radiation effects; Arabidopsis Proteins; Arabidopsis thaliana; Biological Sciences; blue light; Calcium; Calcium - metabolism; Cryptochromes; cytochemistry; Cytoplasm - metabolism; cytosol; Cytosol - metabolism; Drosophila Proteins; Eye Proteins; Flavoproteins - genetics; Flavoproteins - radiation effects; Flowers & plants; hypocotyls; inorganic ions; Kinetics; Light; Luminescence; Luminescent Measurements; mutants; Mutation; Nicotiana; Nicotiana - cytology; Nicotiana - genetics; Nicotiana - metabolism; Nicotiana - radiation effects; Phosphoproteins - genetics; Phosphoproteins - radiation effects; Photoreceptor Cells, Invertebrate; photoreceptors; Photosynthetic Reaction Center Complex Proteins - metabolism; Photosynthetic Reaction Center Complex Proteins - radiation effects; Phototropism; Phototropism - radiation effects; Plant cells; Plant growth; Plants; Plants, Toxic; Protein Serine-Threonine Kinases; Proteins; Receptors; Receptors, G-Protein-Coupled; seedling growth; Seedlings; Signal transduction; Signal Transduction - physiology; Signal Transduction - radiation effects; Spectral sensitivity; stomatal movement; transgenic plants
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.96.23.13554

Blue light regulates plant growth and development, and three photoreceptors, CRY1, CRY2, and NPH1, have been identified. The transduction pathways of these receptors are poorly understood. Transgenic plants containing aequorin have been used to dissect the involvement of these three receptors in the regulation of intracellular Ca(2+). Pulses of blue light induce cytosolic Ca(2+) transients lasting about 80 s in Arabidopsis and tobacco seedlings. Use of organelle-targeted aequorins shows that Ca(2+) increases are limited to the cytoplasm. Blue light treatment of cry1, cry2, and nph1 mutants showed that NPH1, which regulates phototropism, is largely responsible for the Ca(2+) transient. The spectral response of the Ca(2+) transient is similar to that of phototropism, supporting NPH1 involvement. Furthermore, known interactions between red and blue light and between successive blue light pulses on phototropic sensitivity are mirrored in the blue light control of cytosolic Ca(2+) in these seedlings. Our observations raise the possibility that physiological responses regulated by NPH1, such as phototropism, may be transduced through cytosolic Ca(2+).