Recessive and dominant mutations in the ethylene biosynthetic gene ACS5 of Arabidopsis confer cytokinin insensitivity and ethylene overproduction, respectively

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 95; no. 8; pp. 4766 - 4771
• Main Authors: Vogel, J.P. (Carnegie Institution of Washington, Stanford, CA.), Woeste, K.E, Theologis, A, Kieber, J.J
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 14.04.1998; National Acad Sciences; National Academy of Sciences; The National Academy of Sciences
• Subjects: 1-AMINOCYCLOPROPANE-1-CARBOXYLASE SYNTHASE; ACC SYNTHASE; ACS5 GENE; Alleles; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - physiology; ARABIDOPSIS THALIANA; ARN MENSAJERO; ARN MESSAGER; Auxins; Biological Sciences; Biosynthesis; Botany; CIN5 GENE; Crosses, Genetic; Cytokinins; Cytokinins - pharmacology; DNA Primers; DOMINANCE; Enzymes; ETHYLENE; Ethylenes - metabolism; ETILENO; EXPRESION GENICA; EXPRESSION DES GENES; FENOTIPOS; FEUILLE; FLEUR; FLORES; FLOWERS; Flowers & plants; FRUITS; FRUTAS; GENE; GENE EXPRESSION; Gene expression regulation; Gene Expression Regulation, Plant - drug effects; GENE RECESSIF; GENES; GENES RECESIVOS; Genes, Dominant; Genes, Recessive; GENETIC INHERITANCE; Genetic mutation; HEREDITE; HERENCIA GENETICA; HOJAS; Hypocotyl; INDUCED MUTATION; KINETIN; KINETINE; LEAVES; LIASAS; LYASE; LYASES; Lyases - biosynthesis; Lyases - genetics; MESSENGER RNA; MUTACION INDUCIDA; MUTANT; MUTANTES; MUTANTS; Mutation; MUTATION PROVOQUEE; PHENOTYPE; PHENOTYPES; PLANT PRODUCTION; Plants; PLANTULAS; PLANTULE; Polymerase Chain Reaction; PRODUCCION VEGETAL; PRODUCTION VEGETALE; QUINETINA; RECESSIVE GENES; REGULATION; RNA, Messenger - biosynthesis; SEEDLINGS; STRUCTURAL GENES; Transcription, Genetic; Non-programmatic
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.95.8.4766

We identified a set of cytokinin-insensitive mutants by using a screen based on the ethylene-mediated triple response observed after treatment with low levels of cytokinins. One group of these mutants disrupts ACS5, a member of the Arabidopsis gene family that encodes 1-amino-cyclopropane-1-carboxylate synthase, the first enzyme in ethylene biosynthesis. The ACS5 isoform is mainly responsible for the sustained rise in ethylene biosynthesis observed in response to low levels of cytokinin and appears to be regulated primarily by a posttranscriptional mechanism. Furthermore, the dominant ethylene-overproducing mutant eto2 was found to be the result of an alteration of the carboxy terminus of ACS5, suggesting that this domain acts as a negative regulator of ACS5 function